UniProt Accession

 RL7_ECOLI; P0A7K2; P02392; Q2M8S2 

Genbank Protein ID

 V00339; M38301; U00006; U00096; AP009048 

Genbank Nucleotide ID

 CAA23624.1; AAA24573.1; AAC43084.1; AAC76960.1; BAE77334.1 

Protein Name

 50S ribosomal protein L7/L12 

Protein Synonyms/Alias

 L8 

Gene Name

 rplL 

Gene Synonyms/Alias

 b3986; JW3949 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
60	TEFDVILKAAGANKV	acetylation	[1]
66	LKAAGANKVAVIKAV	acetylation	[1]
71	ANKVAVIKAVRGATG	acetylation	[1, 2]
71	ANKVAVIKAVRGATG	methylation	[3]
82	GATGLGLKEAKDLVE	acetylation	[1]
82	GATGLGLKEAKDLVE	methylation	[3]
85	GLGLKEAKDLVESAP	acetylation	[1]
96	ESAPAALKEGVSKDD	acetylation	[1]
101	ALKEGVSKDDAEALK	acetylation	[1]
108	KDDAEALKKALEEAG	acetylation	[1]
109	DDAEALKKALEEAGA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Update on activities at the Universal Protein Resource (UniProt) in 2013.
 e="String">UniProt Consortium.
 Nucleic Acids Res. 2013 Jan;41(Database issue):D43-7. [PMID: 23161681] 

Functional Description

 The binding site for several of the GTPase factors involved in protein synthesis (IF-2, EF-Tu, EF-G and RF3). Is thus essential for accurate translation. Deletion of 1 of the L12 dimers from the ribosome (by deleting the binding site on L10) leads to decreased IF-2 association with the 70S ribosome and decreased stimulation of the GTPase activity of EF-G. 

Sequence Annotation

 REGION 54 121 Required for recruitment of EF-Tu and EF-
 MOD_RES 2 2 N-acetylserine; in L7.
 MOD_RES 82 82 N6-methyllysine; partial.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Methylation; Reference proteome; Ribonucleoprotein; Ribosomal protein. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 121 AA 

Protein Sequence

Gene Ontology

 GO:0022625; C:cytosolic large ribosomal subunit; IDA:EcoliWiki.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0006412; P:translation; IEA:InterPro. 

Interpro

 IPR000206; Ribosomal_L7/12.
 IPR014719; Ribosomal_L7/12_C/ClpS-like.
 IPR013823; Ribosomal_L7/L12_C.
 IPR008932; Ribosomal_L7/L12_oligo. 

Pfam

 PF00542; Ribosomal_L12 

SMART

  

PROSITE

  

PRINTS