CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000477
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tapasin 
Protein Synonyms/Alias
 TPN; TPSN; NGS-17; TAP-associated protein; TAP-binding protein 
Gene Name
 TAPBP 
Gene Synonyms/Alias
 NGS17; TAPA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40ASGKGLAKRPGALLLubiquitination[1, 2, 3]
104VPLPASAKWASGLTPubiquitination[1, 2, 3, 4]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). 
Sequence Annotation
 DOMAIN 292 399 Ig-like C1-type.
 CARBOHYD 253 253 N-linked (GlcNAc...).
 DISULFID 27 91
 DISULFID 115 115 Interchain (with C-57 in PDIA3).
 DISULFID 315 382  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Immunoglobulin domain; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 448 AA 
Protein Sequence
MKSLSLLLAV ALGLATAVSA GPAVIECWFV EDASGKGLAK RPGALLLRQG PGEPPPRPDL 60
DPELYLSVHD PAGALQAAFR RYPRGAPAPH CEMSRFVPLP ASAKWASGLT PAQNCPRALD 120
GAWLMVSISS PVLSLSSLLR PQPEPQQEPV LITMATVVLT VLTHTPAPRV RLGQDALLDL 180
SFAYMPPTSE AASSLAPGPP PFGLEWRRQH LGKGHLLLAA TPGLNGQMPA AQEGAVAFAA 240
WDDDEPWGPW TGNGTFWLPR VQPFQEGTYL ATIHLPYLQG QVTLELAVYK PPKVSLMPAT 300
LARAAPGEAP PELLCLVSHF YPSGGLEVEW ELRGGPGGRS QKAEGQRWLS ALRHHSDGSV 360
SLSGHLQPPP VTTEQHGARY ACRIHHPSLP ASGRSAEVTL EVAGLSGPSL EDSVGLFLSA 420
FLLLGLFKAL GWAAVYLSTC KDSKKVQCST SLYLSLVTLS PHPISKPMEG GCWCGRQNLG 480
LEFTLIWVKT WHYILTVGLF EHAT 504 
Gene Ontology
 GO:0000139; C:Golgi membrane; IDA:UniProtKB.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; TAS:Reactome.
 GO:0042824; C:MHC class I peptide loading complex; NAS:UniProtKB.
 GO:0042288; F:MHC class I protein binding; TAS:UniProtKB.
 GO:0042605; F:peptide antigen binding; TAS:UniProtKB.
 GO:0015433; F:peptide antigen-transporting ATPase activity; TAS:ProtInc.
 GO:0046978; F:TAP1 binding; TAS:UniProtKB.
 GO:0046979; F:TAP2 binding; TAS:UniProtKB.
 GO:0051082; F:unfolded protein binding; TAS:UniProtKB.
 GO:0019885; P:antigen processing and presentation of endogenous peptide antigen via MHC class I; IEA:InterPro.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0006952; P:defense response; IEA:Compara.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0050823; P:peptide antigen stabilization; ISS:UniProtKB.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; NAS:UniProtKB. 
Interpro
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003597; Ig_C1-set.
 IPR008056; Tapasin. 
Pfam
 PF07654; C1-set 
SMART
  
PROSITE
 PS50835; IG_LIKE
 PS00290; IG_MHC 
PRINTS
 PR01669; TAPASIN.