CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002727
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N5-carboxyaminoimidazole ribonucleotide synthase 
Protein Synonyms/Alias
 N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase 
Gene Name
 purK 
Gene Synonyms/Alias
 b0522; JW0511 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
89TQKQLFDKLHLPTAPacetylation[1]
307VHLHWYDKEVRPGRKacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR). 
Sequence Annotation
 DOMAIN 84 267 ATP-grasp.
 NP_BIND 153 156 ATP.
 NP_BIND 237 238 ATP.
 BINDING 80 80 ATP.
 BINDING 120 120 ATP.
 BINDING 131 131 ATP.
 BINDING 161 161 ATP.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MKQVCVLGNG QLGRMLRQAG EPLGIAVWPV GLDAEPAAVP FQQSVITAEI ERWPETALTR 60
ELARHPAFVN RDVFPIIADR LTQKQLFDKL HLPTAPWQLL AERSEWPAVF DRLGELAIVK 120
RRTGGYDGRG QWRLRANETE QLPAECYGEC IVEQGINFSG EVSLVGARGF DGSTVFYPLT 180
HNLHQDGILR TSVAFPQANA QQQAQAEEML SAIMQELGYV GVMAMECFVT PQGLLINELA 240
PRVHNSGHWT QNGASISQFE LHLRAITDLP LPQPVVNNPS VMINLIGSDV NYDWLKLPLV 300
HLHWYDKEVR PGRKVGHLNL TDSDTSRLTA TLEALIPLLP PEYASGVIWA QSKFG 355 
Gene Ontology
 GO:0034028; F:5-(carboxyamino)imidazole ribonucleotide synthase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0004638; F:phosphoribosylaminoimidazole carboxylase activity; IDA:EcoliWiki.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR005875; AIR_COase_ATPase-su.
 IPR011761; ATP-grasp.
 IPR003135; ATP-grasp_carboxylate-amine.
 IPR013816; ATP_grasp_subdomain_2.
 IPR016185; PreATP-grasp_dom.
 IPR011054; Rudment_hybrid_motif. 
Pfam
 PF02222; ATP-grasp 
SMART
  
PROSITE
 PS50975; ATP_GRASP 
PRINTS