UniProt Accession

 YFCG_ECOLI; P77526 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC75362.1; BAA16139.1 

Protein Name

 Disulfide-bond oxidoreductase YfcG 

Protein Synonyms/Alias

 GSH-dependent disulfide-bond oxidoreductase YfcG; GST N1-1; GST-like protein YfcG; Organic hydroperoxidase 

Gene Name

 yfcG 

Gene Synonyms/Alias

 b2302; JW2299 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
30	ELDYRLIKVDLGKGG	acetylation	[1]
35	LIKVDLGKGGQFRPE	acetylation	[1]
146	RLYHVLNKRLENSPW	acetylation	[1]
187	LAMYPAVKNWHERIR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Exhibits a very robust glutathione (GSH)-dependent disulfide-bond reductase activity toward the model substrate, 2- hydroxyethyl disulfide; the actual physiological substrates are not known. Has also a low GSH-dependent hydroperoxidase activity toward cumene hydroperoxide, but does not reduce H(2)O(2), tert- butyl hydroperoxide, benzyl peroxide, or lauroyl peroxide. Exhibits little or no GSH transferase activity with most typical electrophilic substrates, and has no detectable transferase activity using glutathionylspermidine (GspSH) as the nucleophilic substrate. Is involved in defense against oxidative stress, probably via its peroxidase activity. 

Sequence Annotation

 DOMAIN 1 87 GST N-terminal.
 DOMAIN 90 215 GST C-terminal.
 REGION 71 72 Glutathione 1 binding.
 BINDING 11 11 Glutathione 1.
 BINDING 38 38 Glutathione 1.
 BINDING 52 52 Glutathione 1; via amide nitrogen and
 BINDING 132 132 Glutathione 2.  

Keyword

 3D-structure; Complete proteome; Oxidoreductase; Peroxidase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0015036; F:disulfide oxidoreductase activity; IDA:EcoCyc.
 GO:0004601; F:peroxidase activity; IEA:UniProtKB-KW.
 GO:0006979; P:response to oxidative stress; IMP:EcoCyc. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR004046; GST_C.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS