CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029929
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Microtubule-actin cross-linking factor 1 
Protein Synonyms/Alias
  
Gene Name
 Macf1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
4360ELNPEEGKMVEEKYQubiquitination[1]
4711LWENLGEKIAHRQHKacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 5895 AA 
Protein Sequence
MQKGLIDQDT GLVLLESQII MSGLIDPENS EKLSLEEGLT RNFINLPIYQ QLLGLRDSLS 60
LVSRLTGTLG SLSVVEAIEK KIISERLGLK VLEVHLATGG FSLPPSENCI NLEEAFHQGF 120
IASSLHSELQ SHLRSSKNLI DPNTAEKVGL LDLMQRCIIH QESGLKLLPV KQLAGGMVSL 180
KSGRKVSIFR AVQEGLIDRQ VTVRLLEAQL FAGGIVDPRT GHRLTVEEAV RHNLIDQDMA 240
CAILIRQLQT GGIIDTVTGD RMTIDEAVTN NLVAAKIALV ILESLWSFMG LLLPESGEIL 300
PITDALEQGI VSTELAHKIL HNRQQIEALF LPTLTEIWSW EKATESGILD KDLVNNLRSV 360
CIPDMMPHIQ LADSAEQSKV GFAAGKPPVS GPREEGSSHG EKLLFQLMTH SYIHAHTGQR 420
LLLLDQELVE MLTSRDDCQV ILPEVFEIQH QRLNTSEALQ ELYTGTISQI SSAKHPRKPC 480
ESQFLSQNKD YPSQENCTEA KGERSVVGIE CSPAESPERE LFLKEQEAII ENVGSLKVIN 540
KVKLKLQRPL LGSRKEEQAE TLREENISGD PLLVECPEES EGKDLSTEKS KCQTPTKCSF 600
TCHKEQVKTI KDIPSETGTS LIKSQNQMSQ FQVDTSVGLR SEFKSEHDMN VNSLEKELKE 660
ELLVKDGHKQ SQEGQSVADG QTVALEKTDT EDNADEPALL HSSPFEDATL STLSAQLQDG 720
GIFNEETGQK LLLNEAIAQG LVSSHTAVKL MGKLNMFRGF FDSQTCESLT TEEVIDEGLM 780
DEKLLYNVLM SDKAISGILD PRTHSLCSVK EAVAAGLLDK ETATRILEGQ VITGGIVDLK 840
RGKKLSVTLA SNLGLVDTAD QTELINLEKA TKGRGAEKAV KERLIELQME TAGLMDPESK 900
APLTVLQSID RGILEREAAV YLLTKQVLDG GIIHHISGLR LSVDNAFKHG LIGEDMARQL 960
RKVENFIHYQ FFHPQTKEAL SFSEAIKLDL VSPDLKREIQ EIQDFSGNLG DFIYGQKLTL 1020
AKTNKEESLA NKTELPSGVM HGVIDPENCT IIPYSELVKK CRIDTESGWR YLEVIPFSDI 1080
KDEAGNNVLT PPEAIQLGKV DFASALKVLE AQANTGGIID MATGKRVTLA SALEKKLLDE 1140
NMARIIASHQ MLSGGIIDIY SDQRVTLNDA VEKRLISPEL AAMIQVDPLA EQGGTGVCEL 1200
KGDFLRKELL SESSKTPRES YSKEKHEAVL QAGSLCAPEK AGIRGSNGEK AEKGRKISVE 1260
MEGQRQDEKA SSDNKVSASI LSPFGFEGES SYQVSVTHPC SESCDLKPRE ETRSCMKKCA 1320
VVERDKVVTQ IKMVSHVKQS TSGLDAEEAR ERQGRMVSKE QGSHYETAGN LLSERSVRVD 1380
RRVRREMGGE QSVQMSREAA VLSEEELDQE VTIGDEPDSF VKSQSMKMIG NDKGKEAGIE 1440
KDISVVCKIE GFPSQMTSKD ASLTNQDALP FYTEGETKTV NLCSILKPGE KLSQETASTV 1500
QKEPLSSEIP RPERLNSQES DEEPQISDVP HISKGDMAAQ ITTRQETTDV QDLYITSKSS 1560
ETKDKIFPSK NYIEKLHQEI PMDPTRSHKL KEATISTLET EGISYLDSSD IKSLCEDSKA 1620
DHKSCGHQKS KVTTTQAKKS LEVVDLLVRD TEEGSSEDRV GQRGPRVLAS LLPEKLPTRT 1680
VQSENIRQHD AVIPAISEIR EEMALSLPCS VVKVDGKIPK EKHKEILGDE QGPFMAIPSG 1740
KGIEGVNPEP CRATQNVFTR RLCLEHDEKL VSYLSLLRDI EMRTKQIQPL ELNVAELQDL 1800
LGQAKELDRE LKDLSTVVSQ ELECVDRIVI SQPQEVPAQL LKALEKDAKN LQKSLDSVSD 1860
SWSSRFLHLQ SAVEVKKATV LNRHKELQGK LQDLRAWVGR ASLTLNSKGC DTETDADSLS 1920
HTLQPYKDMK QSMAERKSQL DALALDIQLF ISEHPQDLSL QQNQEMLQFL SELQRSFQGL 1980
VEHTAAQKDV VQGHLQQVQQ EVQVKTLQKQ QDTCHKKLED LCNWVGQAER ALERHQGGAS 2040
RQELPALQQN QSDLKDLQGD IQSHSTSFAT AVKDIEGFLE ENQTKLSPQE LTALREKLHQ 2100
AKEQYEVLQE RTRVAQKELE EAVTSALQQE TEKSKAATEL AENKRKIDAL LDWVASVGSS 2160
ERKPQASLPG MEQFSGACLE KQTLAATDGH VDVNQVPETL DRQYELMKAR HQELLSQQQN 2220
FIVATQSVQS FLDQHSHNLT PEERQKLQEK LGELKEQYAA SLARSEAELK QTQALRDELQ 2280
KFLQDHKEFE NWLQQSENEL DSMHKGGSSP EALNSLLKRQ GSFSEDVISH KGDLRFVTIS 2340
GQKVLETENN FEEGQEPSAT RNLVNEKLKD ATERYTTLHS KCIRLGSHLS MLLGQYQQFQ 2400
SSADSLQAWV LTCEASVGKL LSDTVASDPG VLQQQLATTK QLQEELAEHQ VPVEKLQKAA 2460
HDLLDIEGEP ALDCRPIQET TDSISSRFQN LSCSLDERSA LLQKAIAQSQ SVQESMESLL 2520
QSIREVEQNL ERDQVASLSS GVIQEALANN MKLKQDIARQ KSSLEATHDM VTRFMETADS 2580
NSASVLQGKL AELSQRFQQL QLQQQEKESN LKKLLPQAEM FEQLSNKLQQ FMENKSRLLA 2640
SGNQPDQDIA HFSQQIQELT LAMEDQKENL DTLEHLVTTL GSCGFALDLS QHQDKIQNLK 2700
KDFTELQKTV QEREKDASTC QEQLDEFRKL IRTFQKWLKE TEGNVPPAKT FVSAKELEKQ 2760
IEHLKDLISD WESKGALLGE INAKGTALES LIMDITAPDS QAKTGSILPP VGSSVGSVNG 2820
YHTCKDLTEI QCDMFDVNSK YEKLWEVLRE RQESLQTVFS RMEEVQKEAS SVLQWLESKE 2880
EVLKAMDATL SPTKTETVKA QAESNKAFLA ELEQNSPKIQ KVKEALAGLL KTYPNSQEAE 2940
NWKKMQEDLN SRWEKATEVT VARQKQLEES ASHLACFQAA ESQLRPWLME KELMMGVLGP 3000
LSIDPNMLNA QKQQVQFMLK EFEARRQQHE QLNEAAQGIL TGPGDMSPSA SQVHKDLQSI 3060
SQKWVELTDK LNSRSSQIDQ AIVKSTQYQD LLQDLSEKVK AIGQRLSGQS AISTQPEAVK 3120
QQLEETSEIR SDLGQLDNEI KEAQTLCQEL SLLIGEQYLK DELKKRLETV ALPLQGLEDL 3180
AADRMNRLQA ALASTQQFQQ MFDELRTWLD EKQSQQAKNC PISAKLERLQ CQLQENEEFQ 3240
KNLNQHSGSY EVIVAEGEAL LLSVPPGEEK KTLQNQLVEL RSHWEDLSKK TANRQSRLKD 3300
CMQKAQKYQG HVEDLVPWID ECKSKMSELQ VTLDPVQLES SLLRSKAMLN EAEKRRSLLE 3360
ILNSAADILI NSSEIDEDEI RDEKAGLNQN MDAITEELQA KTSSLEEMTQ RLKEFQESFK 3420
NIEKKVEGAK HQLEIFDALG SQACSNKNLE KLKAQQEVLQ ALEPQVDYLR NFTQGLVEDA 3480
PDGSDASPLV HQAEVAQQEF LEVKQRVSSS CLTMENKLEG IGQFHCRVRE MFSQLADLDD 3540
ELDGMGAIGR DTDSLQSQIE DVRLFLNKIQ ALRFDIEDSE AECRKMLEEE GTLDLLGLKR 3600
ELEALNKQCG KLTERGKVRQ EQLELTLGRV EDFYRKLKAL NDAATAAEEG EALQWIVGTE 3660
VDVINQQLAD FKLFQKDQVD PLQVKLQQVN GLGQGLIQSA GKNCDVQGLE HDMDEINTRW 3720
NTLNKKVAQR IAQLQEALLH CGKFQDALEP LLSWLTDTEE LIANQKPPSA EYKVVKAQIQ 3780
EQKLLQRLLD DRKATVDMLQ AEGGRIAQSA ELADREKITG QLESLERRWT DLLSKAAARQ 3840
KQLEDILVLA KQFHETAEPI SDFLSVTEKK LANSEPVGTQ TAKIHQQIIR HKALEEEIEN 3900
HATDVHQAVK IGQSLSSLTC PAEQGIMSEK LDSLQARYSE IQDRCCRKAS LLEQALFNAR 3960
LFGEDEVEVL NWLAEVEDKL STVFVKDYRQ DVLQKQHADH LALNEEIINR KKNVDQAIKN 4020
GQALLKQTTG EEVLLIQEKL DGIKTRYADI TLTSSKALRT LEQARQLATK FHSTYEELTG 4080
WLREAEEELA ASGGQSPTGE QIPQFQQRQK ELKKEVMEHR LVLDTVNEVS HALLELVPWR 4140
AREGLDKLVS DANEQYKLIS DTVGQRVDEI DAAIQRSQQY EQAADAELAW VAETKRKLMA 4200
LGPIRLEQDQ TTAQLQVQKA FSIDIIRHKD SMDELFSHRG EIFSTCGEEQ KAVLQEKTEC 4260
LIQQYEAVSL LNSERYARLE RAQVLVNQFW ETYEELSPWA EETLALIAQL PPPAVDHEQL 4320
RQQQEEMRQL RESIAEHKPH IDKILKIGPQ LKELNPEEGK MVEEKYQKAE NMYAQIKDEV 4380
RQRALALDEA VSQSAQFHDK IEPMLETLEN LSSRLRMPPL IPAEVDKIRE CISDNKSATV 4440
ELEKLQPSFE ALKRRGEELI GRSQGADKDL AAKEIQDKLD QMVFFWEDIK ARSEEREIKF 4500
LDVLELAEKF WYDMAALLTT IKDTQDIVHD LESPGIDPSI IKQQVEAAET IKEETDGLHE 4560
ELEFIRILGA DLIFACGETE KPEVKKSIDE MNNAWENLNK TWKERLEKLE DAMQAAVQYQ 4620
DTLQAMFDWL DNTVIKLCTM PPVGTDLNTV KDQLNEMKEF KVEVYQQQIE MEKLNHQGEL 4680
MLKKATDETD RDIIREPLTE LKHLWENLGE KIAHRQHKLE GALLALGQFQ HALEELMSWL 4740
THTEELLDAQ RPISGDPKVI EVELAKHHVL KNDVLAHQAT VATVNKAGSE LLESSAGDDA 4800
SSLRSRLETM NQCWESVLQK TEEREQQLQS TLQQAQGFHS EIEDFLLELN RMENQLSASK 4860
PTGGLPETAR EQLDTHMELH SQLRAKEEIY NQLLDKGRLM LLSRGDSGSG SKTEQSVALL 4920
EQKWHAVSSK VEERKSKLEE ALSLATEFQN SLQEFINWLT LAEQSLNIAS PPSLILNTVL 4980
SQIEEHKVFA NEVNDHRDQI IELDQTGNQL KFLSQKQDVV LIKNLLVSVQ SRWEKVVQRS 5040
IERGRSLDDA RKRAKQFHEA WKKLIDWLED AESHLDSELE ISNDPDKIKL QLSKHKEFQK 5100
TLGGKQPVYD TTIRTGRALK EKTLLAGDTQ KLDNLLGEVR DKWDTVCGKS VERQHKLEEA 5160
LLFSGQFMDA LQALVDWLYK VEPQLAEDQP VHGDLDLVMN LMDAHKVFQK ELGKRTGTVQ 5220
VLKRSGRELI EGSRDDTTWV KGQLQELSTR WDTVCKLSVS KQSRLEQALK QAEEFRDTVH 5280
MLLEWLSEAE QTLRFRGALP DDTEALQSLI DTHKEFMKKV EEKRVDVNTA VAMGEAILAV 5340
CHPDCITTIK HWITIIRARF EEVLTWAKQH QQRLETALSE LVANAELLEE LLAWIQWAET 5400
TLIQRDQEPI PQNIDRVKAL ITEHQSFMEE MTRKQPDVDR VTKTYKRKSV EPTHAPFMEK 5460
SRSGSRKSLN QPTPPPMPIL SQSEAKNPRI NQLSARWQQV WLLALERQRK LNDALDRLEE 5520
LKEFANFDFD VWRKKYMRWM NHKKSRVMDF FRRIDKDQDG KITRQEFIDG ILASKFPTTK 5580
LEMTAVADIF DRDGDGYIDY YEFVAALHPN KDAYRPTTDA DKIEDEVTRQ VAQCKCAKRF 5640
QVEQIGENKY RFFLGNQFGD SQQLRLVRIL RSTVMVRVGG GWMALDEFLV KNDPCRARGR 5700
TNIELREKFI LPEGASQGMT PFRSRGRRSK PSSRAASPTR SSSSASQSNH SCTSMPSSPA 5760
TPASGTKVIS SSGSKLKRPT PAFHSSRTSL AGDTSNSSSP ASTGAKANRA DPKKSASRPG 5820
SRAGSRAGSR ASSRRGSDAS DFDLLETQSA CSDTSESSAA GGQGSSRRGL TKPSKIPTMS 5880
KKTTTASPRT PGPKR 5895 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:MGI.
 GO:0015630; C:microtubule cytoskeleton; IDA:MGI.
 GO:0003779; F:actin binding; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008017; F:microtubule binding; IDA:MGI.
 GO:0007050; P:cell cycle arrest; IEA:InterPro.
 GO:0006928; P:cellular component movement; IMP:MGI.
 GO:0007163; P:establishment or maintenance of cell polarity; IMP:MGI.
 GO:0001707; P:mesoderm formation; IMP:MGI.
 GO:0006620; P:posttranslational protein targeting to membrane; IMP:MGI.
 GO:0016055; P:Wnt receptor signaling pathway; IMP:MGI. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR003108; GAS2_dom.
 IPR001101; Plectin_repeat.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF13499; EF_hand_5
 PF02187; GAS2
 PF00681; Plectin
 PF00435; Spectrin 
SMART
 SM00054; EFh
 SM00243; GAS2
 SM00250; PLEC
 SM00150; SPEC 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51460; GAR 
PRINTS