CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002923
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 4-hydroxy-tetrahydrodipicolinate synthase 
Protein Synonyms/Alias
 HTPA synthase 
Gene Name
 dapA 
Gene Synonyms/Alias
 b2478; JW2463 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
153ETVGRLAKVKNIIGIacetylation[1]
155VGRLAKVKNIIGIKEacetylation[1]
161VKNIIGIKEATGNLTacetylation[1]
219RDMAQMCKLAAEGHFacetylation[1]
242RLMPLHNKLFVEPNPacetylation[1]
257IPVKWACKELGLVATacetylation[1]
287ETVRAALKHAGLL**acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the condensation of (S)-aspartate-beta- semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA). 
Sequence Annotation
 ACT_SITE 133 133 Proton donor/acceptor.
 ACT_SITE 161 161 Schiff-base intermediate with substrate.
 BINDING 45 45 Pyruvate.
 BINDING 203 203 Pyruvate; via carbonyl oxygen.  
Keyword
 3D-structure; Allosteric enzyme; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Diaminopimelate biosynthesis; Direct protein sequencing; Lyase; Lysine biosynthesis; Reference proteome; Schiff base. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 292 AA 
Protein Sequence
MFTGSIVAIV TPMDEKGNVC RASLKKLIDY HVASGTSAIV SVGTTGESAT LNHDEHADVV 60
MMTLDLADGR IPVIAGTGAN ATAEAISLTQ RFNDSGIVGC LTVTPYYNRP SQEGLYQHFK 120
AIAEHTDLPQ ILYNVPSRTG CDLLPETVGR LAKVKNIIGI KEATGNLTRV NQIKELVSDD 180
FVLLSGDDAS ALDFMQLGGH GVISVTANVA ARDMAQMCKL AAEGHFAEAR VINQRLMPLH 240
NKLFVEPNPI PVKWACKELG LVATDTLRLP MTPITDSGRE TVRAALKHAG LL 292 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0008840; F:4-hydroxy-tetrahydrodipicolinate synthase; IDA:EcoliWiki.
 GO:0016843; F:amine-lyase activity; IEA:HAMAP.
 GO:0019877; P:diaminopimelate biosynthetic process; IDA:EcoliWiki.
 GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:UniProtKB-UniPathway. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR002220; Dihydrodipicolinate_synth-like.
 IPR020625; Dihydrodipicolinate_synth_AS.
 IPR020624; Dihydrodipicolinate_synth_CS.
 IPR005263; Dihydrodipicolinate_synth_DapA. 
Pfam
 PF00701; DHDPS 
SMART
  
PROSITE
 PS00665; DHDPS_1
 PS00666; DHDPS_2 
PRINTS
 PR00146; DHPICSNTHASE.