CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012113
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Non-receptor tyrosine-protein kinase TNK1 
Protein Synonyms/Alias
 CD38 negative kinase 1 
Gene Name
 TNK1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
141LWTLPSGKSVPVAVKubiquitination[1]
260LASPRTIKVADFGLVubiquitination[1]
418SPDSTIWKGQNGRTFubiquitination[1, 2]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction. 
Sequence Annotation
 DOMAIN 116 377 Protein kinase.
 DOMAIN 381 444 SH3.
 NP_BIND 122 130 ATP (By similarity).
 ACT_SITE 245 245 Proton acceptor (By similarity).
 BINDING 148 148 ATP (By similarity).
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 255 255 Phosphoserine.
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 514 514 Phosphothreonine.
 MOD_RES 519 519 Phosphoserine.
 MOD_RES 582 582 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 666 AA 
Protein Sequence
MLPEAGSLWL LKLLRDIQLA QFYWPILEEL NVTRPEHFDF VKPEDLDGIG MGRPAQRRLS 60
EALKRLRSGP KSKNWVYKIL GGFAPEHKEP TLPSDSPRHL PEPEGGLKCL IPEGAVCRGE 120
LLGSGCFGVV HRGLWTLPSG KSVPVAVKSL RVGPEGPMGT ELGDFLREVS VMMNLEHPHV 180
LRLHGLVLGQ PLQMVMELAP LGSLHARLTA PAPTPPLLVA LLCLFLRQLA GAMAYLGARG 240
LVHRDLATRN LLLASPRTIK VADFGLVRPL GGARGRYVMG GPRPIPYAWC APESLRHGAF 300
SSASDVWMFG VTLWEMFSGG EEPWAGVPPY LILQRLEDRA RLPRPPLCSR ALYSLALRCW 360
APHPADRPSF SHLEGLLQEA GPSEACCVRD VTEPGALRME TGDPITVIEG SPDSTIWKGQ 420
NGRTFKVGSF PASAVTLADA GGLPATRPVH RGTPARGDQH PGSIDGDRKK ANLWDAPPAR 480
GQRRNMPLER MKGISRSLES VLSLGPRPTG GGSSPPEIRQ ARAVPQGPPG LPPRPPLSSS 540
SPQPSQPSRE RLPWPKRKPP HNHPMGMPGA RKAAALSGGL LSDPELQRKI MEVELSVHGV 600
THQECQTALG ATGGDVVSAI RNLKVDQLFH LSSRSRADCW RILEHYQWDL SAASRYVLAR 660
P 661 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; NAS:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR001452; SH3_domain.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 
Pfam
 PF07714; Pkinase_Tyr 
SMART
 SM00326; SH3
 SM00219; TyrKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS50002; SH3 
PRINTS
 PR00109; TYRKINASE.