UniProt Accession

 DTX1_HUMAN; Q86Y01; O60630; Q9BS04 

Genbank Protein ID

 AF053700; BC005816; BC048216 

Genbank Nucleotide ID

 AAC06246.1; AAH05816.2; AAH48216.1 

Protein Name

 E3 ubiquitin-protein ligase DTX1 

Protein Synonyms/Alias

 Protein deltex-1; Deltex1; hDTX1 

Gene Name

 DTX1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
404	RRYMQKVKNPPDEDC	ubiquitination	[1]
431	YEGVLRHKGVRPELV	ubiquitination	[1, 2]
472	SLQCPTCKAIYGEKT	ubiquitination	[1, 2]
526	EHPNPGKKFTARGFP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T- cell development, suggesting that it can antagonize NOTCH1. 

Sequence Annotation

 DOMAIN 14 94 WWE 1.
 DOMAIN 95 171 WWE 2.
 ZN_FING 411 472 RING-type.
 MOTIF 230 233 SH3-binding (Potential).  

Keyword

 Complete proteome; Cytoplasm; Ligase; Metal-binding; Notch signaling pathway; Nucleus; Reference proteome; Repeat; SH3-binding; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 620 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0005112; F:Notch binding; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0010001; P:glial cell differentiation; IEA:Compara.
 GO:0045665; P:negative regulation of neuron differentiation; IGI:UniProtKB.
 GO:0045581; P:negative regulation of T cell differentiation; IEA:Compara.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0008593; P:regulation of Notch signaling pathway; IGI:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 

Interpro

 IPR004170; WWE-dom.
 IPR018123; WWE-dom_subgr.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF02825; WWE
 PF13639; zf-RING_2 

SMART

 SM00184; RING
 SM00678; WWE 

PROSITE

 PS50918; WWE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS