UniProt Accession

 VATH_HUMAN; Q9UI12; B3KMR0; Q6PK44; Q9H3E3; Q9Y300 

Genbank Protein ID

 AF113222; AF298777; AF112204; AF132945; AK022345; CH471068; BC025275 

Genbank Nucleotide ID

 AAG39293.1; AAG22809.1; AAF17192.1; AAD27720.1; BAG51072.1; EAW86727.1; AAH25275.1 

Protein Name

 V-type proton ATPase subunit H 

Protein Synonyms/Alias

 V-ATPase subunit H; Nef-binding protein 1; NBP1; Protein VMA13 homolog; V-ATPase 50/57 kDa subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD 

Gene Name

 ATP6V1H 

Gene Synonyms/Alias

 CGI-11 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
22	PTNIIAAKAAEVRAN	ubiquitination	[1, 2]
176	KTQLSSQKLRGSGVA	ubiquitination	[1]
325	LENLEQQKYDDEDIS	ubiquitination	[1]
341	DIKFLLEKLGESVQD	ubiquitination	[1]
360	DEYSSELKSGRLEWS	ubiquitination	[1]
374	SPVHKSEKFWRENAV	ubiquitination	[2]
386	NAVRLNEKNYELLKI	ubiquitination	[2]
396	ELLKILTKLLEVSDD	ubiquitination	[1, 3, 4, 5, 6, 7]
469	HNWEYLGKQLQSEQP	ubiquitination	[4, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. 

Sequence Annotation

 MOD_RES 483 483 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Host-virus interaction; Hydrogen ion transport; Ion transport; Phosphoprotein; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 483 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0000221; C:vacuolar proton-transporting V-type ATPase, V1 domain; NAS:UniProtKB.
 GO:0030234; F:enzyme regulator activity; NAS:UniProtKB.
 GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:InterPro.
 GO:0006200; P:ATP catabolic process; NAS:GOC.
 GO:0015991; P:ATP hydrolysis coupled proton transport; NAS:UniProtKB.
 GO:0006879; P:cellular iron ion homeostasis; TAS:Reactome.
 GO:0006897; P:endocytosis; IDA:UniProtKB.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0051701; P:interaction with host; TAS:Reactome.
 GO:0090382; P:phagosome maturation; TAS:Reactome.
 GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
 GO:0033572; P:transferrin transport; TAS:Reactome.
 GO:0007035; P:vacuolar acidification; NAS:UniProtKB.
 GO:0016032; P:viral reproduction; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR004908; ATPase_V1-cplx_hsu.
 IPR011987; ATPase_V1-cplx_hsu_C. 

Pfam

 PF11698; V-ATPase_H_C
 PF03224; V-ATPase_H_N 

SMART

  

PROSITE

  

PRINTS