CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001397
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Baculoviral IAP repeat-containing protein 6 
Protein Synonyms/Alias
 BIR repeat-containing ubiquitin-conjugating enzyme; BRUCE; Ubiquitin-conjugating BIR domain enzyme apollon; APOLLON 
Gene Name
 Birc6 
Gene Synonyms/Alias
 Kiaa1289 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
95VTSRGTIKVIDGTSGubiquitination[1]
551NPSLTNSKSEKTKEKubiquitination[1]
1652EKQQQLLKLQQQKAKubiquitination[1]
2207EDEAAAAKKPLNGKDubiquitination[1]
2253LDRLYSRKIRKQLVHubiquitination[1]
2293SNKGSSYKLLVEQAKubiquitination[1]
2300KLLVEQAKLKQATSKubiquitination[1]
3162FLDSGPNKAVDSTLKubiquitination[1]
3169KAVDSTLKTRILASEubiquitination[2]
3916EQKDDKEKKNHEEKEubiquitination[1]
4044ITDPSLSKTDSFKRLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. Required for normal placenta development. 
Sequence Annotation
 REPEAT 292 362 BIR.
 REGION 4592 4720 Ubiquitin-conjugating (By similarity).
 ACT_SITE 4691 4691 Glycyl thioester intermediate (By
 MOD_RES 476 476 Phosphoserine.
 MOD_RES 483 483 Phosphoserine.
 MOD_RES 489 489 Phosphoserine.
 MOD_RES 2976 2976 Phosphoserine.  
Keyword
 Alternative splicing; Apoptosis; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Endosome; Golgi apparatus; Ligase; Membrane; Mitosis; Phosphoprotein; Protease inhibitor; Reference proteome; Thiol protease inhibitor; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4882 AA 
Protein Sequence
MVTGCGAAPP GTVTERLPSV IVLSAGRKMA AAAAEASGPS CSSAAAAAGA GAAGVSEWLV 60
LRDGCMRCDA DGLHSLSYHP ALNAILAVTS RGTIKVIDGT SGATLQASAL SAKPGGQVKC 120
QYISAVDKVI FVDDYAVGCR KDLNGILLLD TALQTPVSKQ DDVVQLELPV TEAQQLLSAC 180
IEKIDVSSTE GYDLFITQLK DGLKNTSHET AANHKVAKWA TVTFHLPHHV LKSIASAIVN 240
ELKKINQNVA ALPVASSVMD RLSYLLPSAR PELGVGPGRS VDRALMYSEA NRRETFTSWP 300
HVGYRWAQPD PMAQAGFYHQ PASSGDDRAM CFTCSVCLVC WEPTDEPWSE HERHSPNCPF 360
VKGEHTQNVP LSVTLATSPA QLPSADGADR IACFGSGSCP QFLAAATKRG KICIWDVSKL 420
MKVHLKFEIN AYDPAIVQQL ILSGDPSSGV DSRRPTLAWL EDSSSCSDIP KLEGDSDDLL 480
EDSDSEEHSR SDSVTGHTSQ KEAMEVSLDI TALSILQQPE KLQWEIVANV LEDTVKDLEE 540
LGANPSLTNS KSEKTKEKHQ EQHNIPFPCL LAGGLLTYKS PATSPISSNS HRSLDGLSRT 600
QGESISEQGS TDNESCTNSE LNSPLVRRTL PVLLLYSIKE SDEKAGKIFS QMNNIMSKSL 660
HDDGFTVPQI IEMELDNQEQ LLLQDPPVTY IQQFADAAAS LTSPDSEKWN SVFPKPGALV 720
QCLRLPKFAE EETLCIDSIT PCADGIHLLV GLRTCSVESL SAINQVEALN NLNKLNSALC 780
NRRKGDLESN LAVVNGANIS VIQHESPADV PEHLLIRPEQ RNVVSGGYLV LYKMNYTTRI 840
VTLEEEPVKI QHIKDPQDTI TSLILLPPDI LDNREDDCEE PAEEMQLASK NGIEREKKSD 900
ISTLGHLVVT TQGGYVKVLD LSNFEILAKV EPPKKEGTEE QDTFVSVIYC SGTDRLCACT 960
KGGELHFLQI GGTCDDIDEA DILVDGSLSK GIEPALEGSR PLSNPSSPGI SGVELLVDQP 1020
FTLEILTSLV ELTRFETLTP RFSATVPPCW VEVQQEQQQR RHPQHLHQQH HGDAAQHTRT 1080
WKLQTDSNSW DEHVFELVLP KACMVGHVDF KFVLNSNITS VPQIQVTLLK NKAPGLGKAN 1140
ALNIEVEHNG NPSLVDLNEE MHHMDVEESQ CLRLCPFLED HKEDILCGPV WLASGLDLSG 1200
HAGMLTLTSP KLVKGMAGGK YRSFLIHVKA VSDRGAADEM CSSGLRPVVR LPSLKQQGHK 1260
GYSLASLLAK VAAGKEKSSN VKNENAGGTR KSENLRGCDL LQEVSVTIRR FKKTSICKER 1320
VQRCAMLQFS EFHEKLLNTL CRRSDDGQVT EHAQSLVLDA LCWLAGVHSN GSGSSKEGNE 1380
CLLSKTRKCL SDIVRVCFFE AGRSIAHKCA RFLALCISNG KCEPCQPGFG SVLLKALLDN 1440
MCFLPAAATG GSVYWYFVLL NYVKDEDLAG CSTACAALLT AVSRQLQDRL TPLEALLQTR 1500
YGLYSSPFDP VLFDLEMSGS SWKTVYSSST AVQSDEIDLS DVLSGNGRVS SCTAAEGSFT 1560
SLTGLLEVEP LHFTCVSTSD GTRIERDDAS TFTVSSFGVP PAVGGLSSGT VGEASTALSS 1620
AAQVALQSLS HAMASAEQQL QVLQEKQQQL LKLQQQKAKL EAKLHQTTAA AAAAASAAAA 1680
AAAGPVHNAV PSNPVAAPGF FIHPSDVIPP TPKTTPLFMT PPLTPPNEAV SVVINAELAQ 1740
LFPGSVIDPP AVNLAAQNKN SSKSRMNPLG SGLALAISHA SHFLQPPPHQ SIIIERMHSG 1800
ARRFVTLDFG RPILLTDVLI PTCGDLASLS IDIWTLGEEV DGRRLVVATD ISTHSLILHD 1860
LIPPPVCRFM KITVIGRYGS TNARAKIPLG FYYGHSYILP WESELKLMHD PLRGEGESAS 1920
QPEIDQHLAM MVALQEDIQC RYNLACHRLE ALLQSIDLPP LNSANNAQYF LRKPDKAVEE 1980
DSRVFSAYQD CIQLQLQLNL AHNAVQRLKV AIGASRKLLN ETSGPEDLIQ TSSTEQLRTI 2040
VRYLLDTLLS LLHSSNGHSV PAVLQSTFHA QACEELFKHL CISGTPKIRL HTGLLLVQLC 2100
GGERWWGQFL SNVLQELYNS EQLLIFPQDR VFMLLSCIGQ RSLSNSGVLE SLLNLLDNLL 2160
SPLQPELSMH RRTEGVLDIP MISWVVMLVS RLLDYVATVE DEAAAAKKPL NGKDRERFLT 2220
GNQWSFINNN LHTQNLNRSS KGGSSLDRLY SRKIRKQLVH HKQQLNLLKA KQKALVEQME 2280
KEKIQSNKGS SYKLLVEQAK LKQATSKHFK DLIRLRRTAE WSRSNLDTEV TTTKESPEIE 2340
PLPFTLAHDR CISVVQKLVL FLLSMDFTCH ADLLLFVCKV LARIANATRP TIHLCEIVNE 2400
PQLERLLLLL VGTDFNRGDI SWGGAWAQYS LTCMLQDILA GELLAPVAAE AMEECTVSED 2460
VGATAGDSDD SLQQSPAQLL ETIDEPLTHE IAGTPPLSSL EKDKEIDLEL LQDLMEVDID 2520
PLDIDLEKDP LAAKVFKPIS STWYDYWGAD YGTYNYNPYI GGLGMPVAKP PSNTEKNGSQ 2580
TVSVSVSQAL DARLEVGLEQ QAELMLKMMS TLEADSILQA LTNTSPTFSQ SPTGTDDSLL 2640
GNLQPANQNS QLMIQLSSVP MLNVCFNKLF SMLQVHHVQL ESLLQLWLTL SLNSSSSGNK 2700
ENGADIFLYN ANRIPVISLN QASIASFLTV LAWYPNTLLR TWCLVLHSLT LMTNMQLNSG 2760
SSSSIGIQET TAHLLVSDPN LIHVLVKFLS GTSPHGTNQH SPQVGPTATQ AMQEFLTRLQ 2820
VHLSSTCPQI FSELLLKLIH ILSTERGAFQ TGQGPLDAQV KLLEFTLEQN FEVVSVSTIS 2880
AVIESVTFLV HHYITCSDKV MSRSGSDSSA GARACFGGLF ANLIRPGDAK AVCGEMTRDQ 2940
LMFDLLKLVN ILVQLPLSSN REYSARVSVT TNTTDSVSDE EKVSGGKDVN GSSASTPGSP 3000
ACVADLVLAN QQIMSQILSA LGLCNSSAMA MIIGASGLHL TKHENFHGGL DAISVGDGLF 3060
TILTTLSKKA STVHMMLQPI LTYMACGYMG RQGSLATCQL SEPLLWFILR VLDTSDALKA 3120
FHDMGGVQLI CNNMVTSTRA IVNTARSMVS TIMKFLDSGP NKAVDSTLKT RILASEPDNA 3180
EGIHNFAPLG TITSSSPTAQ PAEVLLQATP PHRRARSAAW SYIFLPEEAW CDLTIHLPSA 3240
VLLKEIHIQP HLASLATCPS SVSVEVSADG VNMLPLSTPV VTSGLTYIKI QLVKAEVASA 3300
VCLRLHRPRD ASTLGLSQIK LLGLTAFGTT SSATVNNPFL PSEDQVSKTS IGWLRLLHHC 3360
LTHISDLEGM MASAAAPTAN LLQTCAALLM SPYCGMHSPN IEVVLVKIGL QSTRIGLKLI 3420
DILLRNCAAS GSDPTDLNSP LLFGRLNGLS SDSTIDILYQ LGTTQDPGTK DRIQALLKWV 3480
SDSAKMAALK RSGRMNYMCP SSSAVEYGLL MPSPSHLHCV AAILWHSYEL LVEYDLPALL 3540
DRELFELLFN WSMSLPCNVV LKKAVDSLLC SMCHIHPNYF SLLMGWMGII PPPVQCHHRL 3600
SMTDDSKKQD LSSSLTDDSK NAQAPLSLTE SHLATLASSS QSPEAIKQLL DSGLPSLLVR 3660
SLASFCFSHI SYSESIAQSV DNSQDKLRRH HVPQHCNKMP ITADLVAPIL RFLTEVGNSH 3720
IMKDWLGGSE VNPLWTALLF LLCHSGSTAG GHNLGAQQSS TRSASHSSAT TTVLTTQQRT 3780
AIENATVAFF LQCISCHPNN QKLMAQVLCE LFQTAPQRGS LPTSGNISGF VRRLFLQLML 3840
EDEKVTMFLQ SPCPLYKGRI NATSHVIQHP MFGAGHKFRT LHLPVSTTLS DVLDRVSDTP 3900
SITAKLISEQ KDDKEKKNHE EKEKVKAENG FQDNYSVVVA SGLKSQSKRA MASTPPRPPS 3960
RRGRTIPDKI GSASSSADAA SKIITVPVFH LFHRLLAGQP LPAEMTLAQL LTLLYDRKLP 4020
QGYRSIDLTV KLGSKVITDP SLSKTDSFKR LHPEKDHGDL VGSCPEDEAL TPSDECMDGV 4080
LDESLLETCP IQSPLQVFAG MGGLALIAER LPMLYPEVIQ QVSAPVIAST TQEKPKDSDQ 4140
FEWVTIEQSG ELVYEAPETI AAEPPPVKSA VQATSPIPAH SLAAFGLFLR LPGYAEVLLK 4200
ERKHAQCLLR LVLGVTDDGE GSHILQSPSA NVLPTLPFHV LRSLFSATPL TTDDGVLLRR 4260
MALEIGALHL ILVCLSALSH HAPRVPNSSL SQTEPQVSNS HNPTSAEEQQ LYWAKGTGFG 4320
TGSTASGWDV EQALTKQRLE EEHVTCLLQV LASYINPMSG AVNGEAQASP ESRAQNSSAL 4380
PSVLLELLSQ SCLIPAMSSY LRNDSVLDMA RHVPLYRALL ELLRAIASCT SMVPLLLPLS 4440
TENGEEEEDE QSECQTSVGT LLAKMKTCVD TYTNRLRSKR ENVKAGVKPD APDQEPEGLA 4500
LLVPDIQRTA EIVHAATANL RQANQEKKLG EYSKKVVMKP KPLSVLKSLE EKYVAVMKKL 4560
QFDTFEMVSE DDDGKLGFKV NYHYMSQVKN ANDANSAARA RRLAQEAVTL STSLPLSSSS 4620
SVFVRCDEER LDIMKVLITG PADTPYANGC FEFDVYFPQD YPSSPPLVNL ETTGGHSVRF 4680
NPNLYNDGKV CLSILNTWHG RPEEKWNPQT SSFLQVLVSV QSLILVAEPY FNEPGYERSR 4740
GTPSGTQSSR EYDGNIRQAT VKWAMLEQIR NPSPCFKEVI HKHFYLKRIE LMAQCEEWIA 4800
DIQQYSSDKR VGRTMSHHAA ALKRHTAQLR EELLKLPCPE GLDPDIEDAS PVCRATAGAE 4860
DTLTHDHVNP SSSKDLPSDF QL 4882 
Gene Ontology
 GO:0005768; C:endosome; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IDA:MGI.
 GO:0005815; C:microtubule organizing center; ISS:UniProtKB.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:MGI.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0060711; P:labyrinthine layer development; IMP:MGI.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0008284; P:positive regulation of cell proliferation; IMP:MGI.
 GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
 GO:0060712; P:spongiotrophoblast layer development; IMP:MGI. 
Interpro
 IPR001370; BIR.
 IPR022103; DUF3643.
 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF00653; BIR
 PF12356; DUF3643
 PF00179; UQ_con 
SMART
 SM00238; BIR 
PROSITE
 PS01282; BIR_REPEAT_1
 PS50143; BIR_REPEAT_2
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS