UniProt Accession

 SAE2_MOUSE; Q9Z1F9; Q3TQN3; Q3U819; Q3U9J5; Q8BVX9 

Genbank Protein ID

 U35833; AK075938; AK146925; AK151765; AK152415; AK163451; AK164826; AK166133; AK168673; AK169168; BC054768 

Genbank Nucleotide ID

 AAD10338.1; BAC36068.1; BAE27536.1; BAE30671.1; BAE31200.1; BAE37349.1; BAE37935.1; BAE38590.1; BAE40523.1; BAE40947.1; AAH54768.1 

Protein Name

 SUMO-activating enzyme subunit 2 

Protein Synonyms/Alias

 Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B 

Gene Name

 Uba2 

Gene Synonyms/Alias

 Sae2; Uble1b 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
65	NRQFLFQKKHVGRSK	ubiquitination	[1]
257	DPVKLFTKLFKDDIR	ubiquitination	[1]
260	KLFTKLFKDDIRYLL	acetylation	[2]
271	RYLLTMDKLWRKRKP	acetylation	[2, 3, 4]
322	SYASLFSKSIETLRV	acetylation	[2]
322	SYASLFSKSIETLRV	ubiquitination	[1]
334	LRVHLAEKGDGAELI	ubiquitination	[1]
407	GLKILSGKIDQCRTI	acetylation	[2]
418	CRTIFLNKQPNPRKK	acetylation	[2]
425	KQPNPRKKLLVPCAL	acetylation	[2]
445	NCYVCASKPEVTVRL	acetylation	[2]
465	TVLTLQDKIVKEKFA	ubiquitination	[1]
503	TEANNPKKLSDFGIR	ubiquitination	[1]
611	DDKARKRKLEENEAA	acetylation	[4]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity). 

Sequence Annotation

 NP_BIND 24 29 ATP (By similarity).
 NP_BIND 56 59 ATP (By similarity).
 NP_BIND 95 96 ATP (By similarity).
 NP_BIND 117 122 ATP (By similarity).
 ACT_SITE 173 173 Glycyl thioester intermediate
 METAL 158 158 Zinc (By similarity).
 METAL 161 161 Zinc (By similarity).
 METAL 439 439 Zinc (By similarity).
 METAL 442 442 Zinc (By similarity).
 BINDING 48 48 ATP (By similarity).
 BINDING 72 72 ATP (By similarity).
 MOD_RES 271 271 N6-acetyllysine (By similarity).
 MOD_RES 505 505 Phosphoserine (By similarity).
 MOD_RES 590 590 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Ligase; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 638 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019948; F:SUMO activating enzyme activity; ISS:UniProtKB.
 GO:0016925; P:protein sumoylation; ISS:UniProtKB. 

Interpro

 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023280; Ub-like_act_enz_cat_cys_dom.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 

Pfam

 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 

SMART

  

PROSITE

 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 

PRINTS