UniProt Accession

 ACSA_ECOLI; P27550; Q2M6N5 

Genbank Protein ID

 U00006; U00096; AP009048; M87509 

Genbank Nucleotide ID

 AAC43163.1; AAC77039.1; BAE78071.1; AAA24715.1 

Protein Name

 Acetyl-coenzyme A synthetase 

Protein Synonyms/Alias

 AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme 

Gene Name

 acs 

Gene Synonyms/Alias

 yfaC; b4069; JW4030 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
50	GKILDWIKPYQKVKN	acetylation	[1]
106	GDDASQSKHISYKEL	acetylation	[1]
111	QSKHISYKELHRDVC	acetylation	[1]
130	TLLELGIKKGDVVAI	acetylation	[1]
200	GRSIPLKKNVDDALK	acetylation	[1]
207	KNVDDALKNPNVTSV	acetylation	[1]
221	VEHVVVLKRTGGKID	acetylation	[1]
370	ALMAEGDKAIEGTDR	acetylation	[1]
400	EAWEWYWKKIGNEKC	acetylation	[1]
609	LPKTRSGKIMRRILR	acetylation	[2, 3]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. Acs undergoes a two-step reaction. In the firs thalf reaction, Acs combines acetate with ATP to form acetyl- adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. 

Sequence Annotation

 REGION 191 194 Coenzyme A (By similarity).
 REGION 411 416 Substrate binding (By similarity).
 ACT_SITE 517 517 By similarity.
 METAL 537 537 Magnesium; via carbonyl oxygen (By
 METAL 539 539 Magnesium; via carbonyl oxygen (By
 METAL 542 542 Magnesium; via carbonyl oxygen (By
 BINDING 311 311 Coenzyme A (By similarity).
 BINDING 335 335 Coenzyme A (By similarity).
 BINDING 387 387 Substrate; via amide nitrogen (By
 BINDING 500 500 Substrate (By similarity).
 BINDING 515 515 Substrate (By similarity).
 BINDING 523 523 Coenzyme A (By similarity).
 BINDING 526 526 Substrate (By similarity).
 BINDING 584 584 Coenzyme A (By similarity).
 MOD_RES 609 609 N6-acetyllysine.  

Keyword

 Acetylation; ATP-binding; Complete proteome; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 652 AA 

Protein Sequence

Gene Ontology

 GO:0003987; F:acetate-CoA ligase activity; IDA:EcoCyc.
 GO:0016208; F:AMP binding; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050218; F:propionate-CoA ligase activity; IDA:EcoCyc.
 GO:0045733; P:acetate catabolic process; IMP:EcoCyc.
 GO:0019427; P:acetyl-CoA biosynthetic process from acetate; IEA:HAMAP.
 GO:0006935; P:chemotaxis; IEA:HAMAP. 

Interpro

 IPR011904; Ac_CoA_lig.
 IPR024597; Acyl-CoA_synth_DUF3448.
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig.
 IPR025110; DUF4009. 

Pfam

 PF00501; AMP-binding
 PF11930; DUF3448
 PF13193; DUF4009 

SMART

  

PROSITE

 PS00455; AMP_BINDING 

PRINTS