CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015914
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger FYVE domain-containing protein 16 
Protein Synonyms/Alias
 Endofin; Endosome-associated FYVE domain protein 
Gene Name
 ZFYVE16 
Gene Synonyms/Alias
 KIAA0305 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
86SLNEKTLKGLTSIQNubiquitination[1]
95LTSIQNEKNVTGLDLubiquitination[1, 2, 3]
205NREIGGIKELGIKVDubiquitination[4]
228YSGTENLKDKKIFNQacetylation[5]
259SSKMFHAKDKLQHKSubiquitination[3]
261KMFHAKDKLQHKSQPubiquitination[3]
455IEGMEDRKIDPDQTVubiquitination[6]
558EENVNDSKSQMNQIDubiquitination[1, 2, 3]
614NGLSLGEKSTIPVQQubiquitination[1, 3]
627QQGLPTSKSEITNQLubiquitination[1, 2, 3]
650SVGGARPKQLFSLPSubiquitination[3, 4, 7]
806VCYETISKAQAFERMubiquitination[2]
1202LRLGAEYKAYPAPLTubiquitination[2, 3, 4, 8]
1257EMGKSCIKIPRKKYSubiquitination[1, 2, 3, 4, 7, 8, 9, 10]
1336TSSGFLAKSSIVEDGubiquitination[1, 2, 3]
1372DFKITCGKVDAVDLRubiquitination[2, 10]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [10] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes. 
Sequence Annotation
 ZN_FING 747 805 FYVE-type.
 MOD_RES 446 446 Phosphoserine.
 MOD_RES 815 815 Phosphoserine.
 MOD_RES 845 845 Phosphoserine.
 MOD_RES 939 939 Phosphoserine.
 MOD_RES 946 946 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Endosome; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1539 AA 
Protein Sequence
MDSYFKAAVS DLDKLLDDFE QNPDEQDYLQ DVQNAYDSNH CSVSSELASS QRTSLLPKDQ 60
ECVNSCASSE TSYGTNESSL NEKTLKGLTS IQNEKNVTGL DLLSSVDGGT SDEIQPLYMG 120
RCSKPICDLI SDMGNLVHAT NSEEDIKKLL PDDFKSNADS LIGLDLSSVS DTPCVSSTDH 180
DSDTVREQQN DISSELQNRE IGGIKELGIK VDTTLSDSYN YSGTENLKDK KIFNQLESIV 240
DFNMSSALTR QSSKMFHAKD KLQHKSQPCG LLKDVGLVKE EVDVAVITAA ECLKEEGKTS 300
ALTCSLPKNE DLCLNDSNSR DENFKLPDFS FQEDKTVIKQ SAQEDSKSLD LKDNDVIQDS 360
SSALHVSSKD VPSSLSCLPA SGSMCGSLIE SKARGDFLPQ HEHKDNIQDA VTIHEEIQNS 420
VVLGGEPFKE NDLLKQEKCK SILLQSLIEG MEDRKIDPDQ TVIRAESLDG GDTSSTVVES 480
QEGLSGTHVP ESSDCCEGFI NTFSSNDMDG QDLDYFNIDE GAKSGPLISD AELDAFLTEQ 540
YLQTTNIKSF EENVNDSKSQ MNQIDMKGLD DGNINNIYFN AEAGAIGESH GINIICEIVD 600
KQNTIENGLS LGEKSTIPVQ QGLPTSKSEI TNQLSVSDIN SQSVGGARPK QLFSLPSRTR 660
SSKDLNKPDV PDTIESEPST ADTVVPITCA IDSTADPQVS FNSNYIDIES NSEGGSSFVT 720
ANEDSVPENT CKEGLVLGQK QPTWVPDSEA PNCMNCQVKF TFTKRRHHCR ACGKVFCGVC 780
CNRKCKLQYL EKEARVCVVC YETISKAQAF ERMMSPTGSN LKSNHSDECT TVQPPQENQT 840
SSIPSPATLP VSALKQPGVE GLCSKEQKRV WFADGILPNG EVADTTKLSS GSKRCSEDFS 900
PLSPDVPMTV NTVDHSHSTT VEKPNNETGD ITRNEIIQSP ISQVPSVEKL SMNTGNEGLP 960
TSGSFTLDDD VFAETEEPSS PTGVLVNSNL PIASISDYRL LCDINKYVCN KISLLPNDED 1020
SLPPLLVASG EKGSVPVVEE HPSHEQIILL LEGESFHPVT FVLNANLLVN VKFIFYSSDK 1080
YWYFSTNGLH GLGQAEIIIL LLCLPNEDTI PKDIFRLFIT IYKDALKGKY IENLDNITFT 1140
ESFLSSKDHG GFLFITPTFQ KLDDLSLPSN PFLCGILIQK LEIPWAKVFP MRLMLRLGAE 1200
YKAYPAPLTS IRGRKPLFGE IGHTIMNLLV DLRNYQYTLH NIDQLLIHME MGKSCIKIPR 1260
KKYSDVMKVL NSSNEHVISI GASFSTEADS HLVCIQNDGI YETQANSATG HPRKVTGASF 1320
VVFNGALKTS SGFLAKSSIV EDGLMVQITP ETMNGLRLAL REQKDFKITC GKVDAVDLRE 1380
YVDICWVDAE EKGNKGVISS VDGISLQGFP SEKIKLEADF ETDEKIVKCT EVFYFLKDQD 1440
LSILSTSYQF AKEIAMACSA ALCPHLKTLK SNGMNKIGLR VSIDTDMVEF QAGSEGQLLP 1500
QHYLNDLDSA LIPVIHGGTS NSSLPLEIEL VFFIIEHLF 1539 
Gene Ontology
 GO:0031901; C:early endosome membrane; TAS:Reactome.
 GO:0005545; F:1-phosphatidylinositol binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; IDA:UniProtKB.
 GO:0008565; F:protein transporter activity; IMP:UniProtKB.
 GO:0030509; P:BMP signaling pathway; TAS:Reactome.
 GO:0016197; P:endosomal transport; IMP:UniProtKB.
 GO:0006622; P:protein targeting to lysosome; IMP:UniProtKB.
 GO:0030100; P:regulation of endocytosis; TAS:UniProtKB.
 GO:0016050; P:vesicle organization; NAS:UniProtKB. 
Interpro
 IPR022557; DUF3480.
 IPR000306; Znf_FYVE.
 IPR017455; Znf_FYVE-rel.
 IPR011011; Znf_FYVE_PHD.
 IPR017165; Znf_FYVE_SARA/endofin.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF11979; DUF3480
 PF01363; FYVE 
SMART
 SM00064; FYVE 
PROSITE
 PS50178; ZF_FYVE 
PRINTS