UniProt Accession

 DDLB_ECOLI; P07862 

Genbank Protein ID

 M14029; K02668; X52644; X55034; U00096; AP009048 

Genbank Nucleotide ID

 AAA23672.1; AAA23815.1; CAA36869.1; CAA38869.1; AAC73203.1; BAB96660.1 

Protein Name

 D-alanine--D-alanine ligase B 

Protein Synonyms/Alias

 D-Ala-D-Ala ligase B; D-alanylalanine synthetase B 

Gene Name

 ddlB 

Gene Synonyms/Alias

 ddl; b0092; JW0090 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
43	DAYPVDPKEVDVTQL	acetylation	[1]
124	LTRAEFEKGLSDKQL	acetylation	[1]
129	FEKGLSDKQLAEISA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Cell wall formation. 

Sequence Annotation

 DOMAIN 101 303 ATP-grasp.
 NP_BIND 134 189 ATP (By similarity).
 ACT_SITE 15 15
 ACT_SITE 150 150
 ACT_SITE 281 281
 METAL 257 257 Magnesium or manganese 1 (By similarity).
 METAL 270 270 Magnesium or manganese 1 (By similarity).
 METAL 270 270 Magnesium or manganese 2 (By similarity).
 METAL 272 272 Magnesium or manganese 2 (By similarity).  

Keyword

 3D-structure; ATP-binding; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Peptidoglycan synthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 306 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0008716; F:D-alanine-D-alanine ligase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0009252; P:peptidoglycan biosynthetic process; IMP:EcoCyc.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. 

Interpro

 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR000291; D-Ala_lig_Van_CS.
 IPR005905; D_ala_D_ala.
 IPR011095; Dala_Dala_lig_C.
 IPR011127; Dala_Dala_lig_N.
 IPR016185; PreATP-grasp_dom. 

Pfam

 PF07478; Dala_Dala_lig_C
 PF01820; Dala_Dala_lig_N 

SMART

  

PROSITE

 PS50975; ATP_GRASP
 PS00843; DALA_DALA_LIGASE_1
 PS00844; DALA_DALA_LIGASE_2 

PRINTS