| Tag | Content |
|---|
| CPLM ID | CPLM-002050 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Gag polyprotein |
Protein Synonyms/Alias | Pr65gag; Core polyprotein; Matrix protein p15; MA; RNA-binding phosphoprotein p12; pp12; Capsid protein p30; CA; Nucleocapsid protein p10; NC-gag |
Gene Name | gag |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Moloney murine leukemia virus (isolate Shinnick) (MoMLV) |
NCBI Taxa ID | 928306 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 408 | SAPDIGRKLERLEDL | sumoylation | [1] | | 416 | LERLEDLKNKTLGDL | sumoylation | [1] | | 418 | RLEDLKNKTLGDLVR | sumoylation | [1] | | 429 | DLVREAEKIFNKRET | sumoylation | [1] | | 433 | EAEKIFNKRETPEER | sumoylation | [1] |
|
Reference | [1] Interaction of moloney murine leukemia virus capsid with Ubc9 and PIASy mediates SUMO-1 addition required early in infection. Yueh A, Leung J, Bhattacharyya S, Perrone LA, de los Santos K, Pu SY, Goff SP. J Virol. 2006 Jan;80(1):342-52. [ PMID: 16352559] |
Functional Description | Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release. |
Sequence Annotation | ZN_FING 502 519 CCHC-type.
REGION 345 393 Interaction with mouse PIAS4.
REGION 430 435 Interaction with mouse UBE2I.
MOTIF 111 114 PTAP/PSAP motif.
MOTIF 130 134 LYPX(n)L motif.
MOTIF 162 165 PPXY motif.
MOD_RES 192 192 Phosphoserine; by host (Probable).
LIPID 2 2 N-myristoyl glycine; by host. |
Keyword | 3D-structure; Alternative initiation; Capsid protein; Coiled coil; Complete proteome; Direct protein sequencing; Host cell membrane; Host endosome; Host membrane; Host-virus interaction; Lipoprotein; Membrane; Metal-binding; Myristate; Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation; Viral budding; Viral budding via the host ESCRT complexes; Viral matrix protein; Viral nucleoprotein; Virion; Virus exit from host cell; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 538 AA |
Protein Sequence | MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW PRDGTFNRDL 60
ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF VHPKPPPPLP PSAPSLPLEP 120
PRSTPPRSSL YPALTPSLGA KPKPQVLSDS GGPLIDLLTE DPPPYRDPRP PPSDRDGNGG 180
EATPAGEAPD PSPMASRLRG RREPPVADST TSQAFPLRAG GNGQLQYWPF SSSDLYNWKN 240
NNPSFSEDPG KLTALIESVL ITHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGDDGR 300
PTQLPNEVDA AFPLERPDWD YTTQAGRNHL VHYRQLLLAG LQNAGRSPTN LAKVKGITQG 360
PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVSMSFIWQ SAPDIGRKLE RLEDLKNKTL 420
GDLVREAEKI FNKRETPEER EERIRRETEE KEERRRTEDE QKEKERDRRR HREMSKLLAT 480
VVSGQKQDRQ GGERRRSQLD RDQCAYCKEK GHWAKDCPKK PRGPRGPRPQ TSLLTLDD 538 |
Gene Ontology | GO:0044185; C:host cell late endosome membrane; IEA:UniProtKB-SubCell. GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell. GO:0072494; C:host multivesicular body; IEA:UniProtKB-SubCell. GO:0016020; C:membrane; IEA:UniProtKB-KW. GO:0019028; C:viral capsid; IEA:UniProtKB-KW. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0005198; F:structural molecule activity; IEA:InterPro. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0019076; P:viral release from host cell; IEA:UniProtKB-KW. GO:0019068; P:virion assembly; IEA:InterPro. GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |