UniProt Accession

 HNRH1_HUMAN; P31943; B3KW86; D3DWQ2; Q6IBM4 

Genbank Protein ID

 L22009; CR456778; AK124530; CH471165; CH471165; BC001348 

Genbank Nucleotide ID

 AAA91346.1; CAG33059.1; BAG54048.1; EAW53807.1; EAW53808.1; AAH01348.1 

Protein Name

 Heterogeneous nuclear ribonucleoprotein H 

Protein Synonyms/Alias

 hnRNP H; Heterogeneous nuclear ribonucleoprotein H, N-terminally processed 

Gene Name

 HNRNPH1 

Gene Synonyms/Alias

 HNRPH; HNRPH1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
35	QRFFSDCKIQNGAQG	ubiquitination	[1, 2, 3, 4]
87	HRYVEVFKSNNVEMD	ubiquitination	[1, 4]
98	VEMDWVLKHTGPNSP	ubiquitination	[1, 3, 4, 5]
167	ASQEIAEKALKKHKE	acetylation	[6]
167	ASQEIAEKALKKHKE	ubiquitination	[1, 3, 4, 5, 7]
185	HRYIEIFKSSRAEVR	ubiquitination	[1, 2, 3, 4, 5]
200	THYDPPRKLMAMQRP	ubiquitination	[5]
349	VAAMSKDKANMQHRY	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865] 

Functional Description

 This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG). 

Sequence Annotation

 DOMAIN 11 90 RRM 1.
 DOMAIN 111 188 RRM 2.
 REPEAT 234 249 1-1.
 DOMAIN 289 364 RRM 3.
 REPEAT 354 372 2-1.
 REPEAT 374 392 2-2.
 REPEAT 418 433 1-2.
 REGION 234 433 2 X 16 AA Gly-rich approximate repeats.
 REGION 354 392 2 X 19 AA perfect repeats.
 MOD_RES 1 1 N-acetylmethionine; in Heterogeneous
 MOD_RES 2 2 N-acetylmethionine; in Heterogeneous
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 63 63 Phosphoserine.
 MOD_RES 104 104 Phosphoserine (By similarity).
 MOD_RES 107 107 Phosphothreonine (By similarity).
 MOD_RES 233 233 Dimethylated arginine; alternate.
 MOD_RES 233 233 Omega-N-methylarginine; alternate.
 MOD_RES 246 246 Phosphotyrosine.
 MOD_RES 310 310 Phosphoserine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 449 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0008266; F:poly(U) RNA binding; TAS:ProtInc.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB.
 GO:0043484; P:regulation of RNA splicing; IDA:UniProtKB. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR012996; Znf_CHHC. 

Pfam

 PF08080; zf-RNPHF 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS