CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007053
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA mismatch repair protein MLH1 
Protein Synonyms/Alias
 MutL protein homolog 1; Post meiotic segregation protein 2 
Gene Name
 MLH1 
Gene Synonyms/Alias
 PMS2; YMR167W; YM8520.16 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
446SHSQEAEKLTLNESEacetylation[1]
496KNALPISKDGYIRVPacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for DNA mismatch repair (MMR), correcting base- base mismatches and insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of different MutL heterodimers that form a ternary complex with the MutS heterodimers, which initially recognize the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in maintaining the genetic stability of simple sequence repeats, the repair of heteroduplex sites present in meiotic recombination intermediates, and the promotion of meiotic crossing-over. 
Sequence Annotation
 REGION 1 335 DNA- and ATP-binding.
 REGION 501 756 Interaction with PMS1.
 MOD_RES 441 441 Phosphoserine; by ATM or ATR.  
Keyword
 3D-structure; Complete proteome; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 769 AA 
Protein Sequence
MSLRIKALDA SVVNKIAAGE IIISPVNALK EMMENSIDAN ATMIDILVKE GGIKVLQITD 60
NGSGINKADL PILCERFTTS KLQKFEDLSQ IQTYGFRGEA LASISHVARV TVTTKVKEDR 120
CAWRVSYAEG KMLESPKPVA GKDGTTILVE DLFFNIPSRL RALRSHNDEY SKILDVVGRY 180
AIHSKDIGFS CKKFGDSNYS LSVKPSYTVQ DRIRTVFNKS VASNLITFHI SKVEDLNLES 240
VDGKVCNLNF ISKKSISPIF FINNRLVTCD LLRRALNSVY SNYLPKGNRP FIYLGIVIDP 300
AAVDVNVHPT KREVRFLSQD EIIEKIANQL HAELSAIDTS RTFKASSIST NKPESLIPFN 360
DTIESDRNRK SLRQAQVVEN SYTTANSQLR KAKRQENKLV RIDASQAKIT SFLSSSQQFN 420
FEGSSTKRQL SEPKVTNVSH SQEAEKLTLN ESEQPRDANT INDNDLKDQP KKKQKLGDYK 480
VPSIADDEKN ALPISKDGYI RVPKERVNVN LTSIKKLREK VDDSIHRELT DIFANLNYVG 540
VVDEERRLAA IQHDLKLFLI DYGSVCYELF YQIGLTDFAN FGKINLQSTN VSDDIVLYNL 600
LSEFDELNDD ASKEKIISKI WDMSSMLNEY YSIELVNDGL DNDLKSVKLK SLPLLLKGYI 660
PSLVKLPFFI YRLGKEVDWE DEQECLDGIL REIALLYIPD MVPKVDTSDA SLSEDEKAQF 720
INRKEHISSL LEHVLFPCIK RRFLAPRHIL KDVVEIANLP DLYKVFERC 769 
Gene Ontology
 GO:0005712; C:chiasma; IBA:RefGenome.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0032389; C:MutLalpha complex; IPI:SGD.
 GO:0032390; C:MutLbeta complex; IPI:SGD.
 GO:0000795; C:synaptonemal complex; IBA:RefGenome.
 GO:0005524; F:ATP binding; IMP:SGD.
 GO:0016887; F:ATPase activity; IMP:SGD.
 GO:0030983; F:mismatched DNA binding; IEA:InterPro.
 GO:0000713; P:meiotic heteroduplex formation; IMP:SGD.
 GO:0000710; P:meiotic mismatch repair; IMP:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IMP:SGD. 
Interpro
 IPR002099; DNA_mismatch_repair.
 IPR013507; DNA_mismatch_repair_C.
 IPR014762; DNA_mismatch_repair_CS.
 IPR011186; DNA_mismatch_repair_MLH1.
 IPR014763; DNA_mismatch_repair_N.
 IPR003594; HATPase_ATP-bd.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF01119; DNA_mis_repair 
SMART
 SM00387; HATPase_c 
PROSITE
 PS00058; DNA_MISMATCH_REPAIR_1 
PRINTS