CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019925
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sarcosine dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 SarDH 
Gene Name
 Sardh 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
278CAGVWASKVGRMAGVacetylation[1, 2, 3, 4]
278CAGVWASKVGRMAGVsuccinylation[3]
286VGRMAGVKVPLVAMHacetylation[4]
374NRVPVLEKTGIKSTVacetylation[1, 2, 3, 4, 5]
374NRVPVLEKTGIKSTVsuccinylation[3]
374NRVPVLEKTGIKSTVubiquitination[6]
378VLEKTGIKSTVCGPEacetylation[2, 3]
378VLEKTGIKSTVCGPEsuccinylation[3]
392ESFTPDHKPLMGEAPacetylation[1, 2, 3, 4]
392ESFTPDHKPLMGEAPsuccinylation[3]
392ESFTPDHKPLMGEAPubiquitination[6]
467RSHESYAKNYSVVFPubiquitination[6]
535AYGNQAHKDYTYSRLacetylation[3]
535AYGNQAHKDYTYSRLsuccinylation[3]
560PHHHMIQKECLACRGacetylation[1, 2, 4]
776IDSLSIEKGYRHWHAacetylation[1, 2, 4]
776IDSLSIEKGYRHWHAubiquitination[6]
803LAFTCKLKTSVPFLGacetylation[2, 3, 4]
803LAFTCKLKTSVPFLGsuccinylation[3]
803LAFTCKLKTSVPFLGubiquitination[6]
816LGREALEKQRATGLRacetylation[1, 2, 4]
885PVSLDFVKNGEYALEacetylation[1, 2, 3, 4, 5, 7, 8]
885PVSLDFVKNGEYALEsuccinylation[3]
885PVSLDFVKNGEYALEubiquitination[6]
905YAAQVHLKSPFDPDNacetylation[1, 2, 3, 4]
905YAAQVHLKSPFDPDNsuccinylation[3]
913SPFDPDNKRVKGIY*acetylation[2, 3]
913SPFDPDNKRVKGIY*succinylation[3]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758
Functional Description
  
Sequence Annotation
 MOD_RES 109 109 Tele-8alpha-FAD histidine (By
 MOD_RES 778 778 Phosphotyrosine (By similarity).
 MOD_RES 885 885 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 919 AA 
Protein Sequence
MASLSRVLRV AATCPRGRAA WNLGLQPLAT EARPTTEKSV PYQRTLKEEA QGASVVPQGP 60
SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGAVLLERE RLTSGTTWHT AGLLWQLRPS 120
DVEVELLAHT RQVVSRDLEE ETGLHTGWIQ NGGLFIASNQ QRLNEYKRLM SLGKAYGIES 180
HVLSPAETKS LYPLMNVDDL YGTLYVPQDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG 240
IRVRTDDFGV RRVAAVETEH GSIQTPCVVN CAGVWASKVG RMAGVKVPLV AMHHAYVVTE 300
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWE EVSDKFAFGL FDLDWDVFTQ 360
HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG 420
CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHTRWIRER SHESYAKNYS VVFPHDEPLA 480
GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG NQAHKDYTYS 540
RLLGDEYTFD FPPHHHMIQK ECLACRGAAA VFNMSYFGKF YLLGVDARKA ADWLFSADVN 600
RPPGSTVYTC MLNQRGGTES DLTVSRLAPG TQASPLVPAF EGDCYYLAVG GAVAQHNWSH 660
INTVLQDQEF RCQLMDSSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG 720
HLVRAIRLSF VGELGWELHV PRASCLPVYR AVMAAGARHG LVNAGYRAID SLSIEKGYRH 780
WHADLRPDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLICLT VEEEVPMFGL 840
EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGEYA LERMGVTYAA 900
QVHLKSPFDP DNKRVKGIY 919 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0004047; F:aminomethyltransferase activity; IEA:InterPro.
 GO:0008480; F:sarcosine dehydrogenase activity; IEA:EC.
 GO:0006546; P:glycine catabolic process; IEA:InterPro. 
Interpro
 IPR006076; FAD-dep_OxRdtase.
 IPR013977; GCV_T_C.
 IPR006222; GCV_T_N.
 IPR027266; TrmE/GcvT_dom1. 
Pfam
 PF01266; DAO
 PF01571; GCV_T
 PF08669; GCV_T_C 
SMART
  
PROSITE
  
PRINTS