UniProt Accession

 B4DJ93_HUMAN; B4DJ93 

Genbank Protein ID

 AP000302; AP000303; AK295980 

Genbank Nucleotide ID

 BAG58755.1 

Protein Name

 Phosphoribosylformylglycinamidine cyclo-ligase 

Protein Synonyms/Alias

 cDNA FLJ51866, highly similar to Trifunctional purine biosynthetic protein adenosine-3 

Gene Name

 GART 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MLVKKIQPLAKA	ubiquitination	[1]
11	KKIQPLAKATSRSGC	ubiquitination	[1, 2, 3]
19	ATSRSGCKVDLGGFA	ubiquitination	[1, 3, 4]
32	FAGLFDLKAAGFKDP	ubiquitination	[1, 2]
37	DLKAAGFKDPLLASG	ubiquitination	[1, 2, 3, 5, 6]
51	GTDGVGTKLKIAQLC	ubiquitination	[1]
53	DGVGTKLKIAQLCNK	ubiquitination	[1]
109	AVVAGIAKACGKAGC	ubiquitination	[1, 3]
150	GAMERDQKLPHLERI	ubiquitination	[1, 3]
185	LVRKIVAKSSLQYSS	ubiquitination	[1]
228	VLRSGHVKAFAHITG	ubiquitination	[1, 4]
249	IPRVLPEKLGVDLDA	ubiquitination	[1, 2, 3, 5, 6]
295	GAVLVVSKEQTEQIL	ubiquitination	[1, 4]
309	LRDIQQHKEEAWVIG	ubiquitination	[1, 6]
331	GSPRVKVKNLIESMQ	ubiquitination	[1, 2]
357	TNHFSFEKKKARVAV	ubiquitination	[1]
358	NHFSFEKKKARVAVL	ubiquitination	[1]
404	AAVAGLDKAERAGIP	acetylation	[3]
404	AAVAGLDKAERAGIP	ubiquitination	[1, 3]
418	PTRVINHKLYKNRVE	ubiquitination	[1]
458	LSGPFVQKWNGKMLN	ubiquitination	[1]
462	FVQKWNGKMLNIHPS	ubiquitination	[1, 2, 4]
560	NGKICWVKEE*****	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 562 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0008168; F:methyltransferase activity; IEA:InterPro.
 GO:0004641; F:phosphoribosylformylglycinamidine cyclo-ligase activity; IEA:InterPro.
 GO:0004644; F:phosphoribosylglycinamide formyltransferase activity; IEA:InterPro.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:InterPro.
 GO:0032259; P:methylation; IEA:GOC. 

Interpro

 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR002376; Formyl_transf_N.
 IPR001555; GART_AS.
 IPR004733; PurM_cligase.
 IPR016188; PurM_N-like.
 IPR004607; PurN_trans. 

Pfam

 PF00586; AIRS
 PF02769; AIRS_C
 PF00551; Formyl_trans_N 

SMART

  

PROSITE

 PS00373; GART 

PRINTS