CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013868
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leucine-rich repeat serine/threonine-protein kinase 2 
Protein Synonyms/Alias
  
Gene Name
 Lrrk2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1833LLDELMKKAEEGDLLubiquitination[1]
1963DRLLQQDKASLTRTLubiquitination[1]
2091DELAIQGKLPDPVKEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Phosphorylates PRDX3 (By similarity). May also have GTPase activity (By similarity). Has a regulatory role in autophagy induction through a calcium-dependent activation of the CaMKK/AMPK signaling pathway (By similarity). The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes (By similarity). 
Sequence Annotation
 REPEAT 983 1004 LRR 1.
 REPEAT 1012 1033 LRR 2.
 REPEAT 1036 1057 LRR 3.
 REPEAT 1059 1080 LRR 4.
 REPEAT 1084 1105 LRR 5.
 REPEAT 1108 1129 LRR 6.
 REPEAT 1130 1151 LRR 7.
 REPEAT 1174 1195 LRR 8.
 REPEAT 1197 1218 LRR 9.
 REPEAT 1221 1242 LRR 10.
 REPEAT 1246 1267 LRR 11.
 REPEAT 1269 1291 LRR 12.
 DOMAIN 1328 1511 Roc.
 DOMAIN 1879 2146 Protein kinase.
 NP_BIND 1341 1348 GTP (Potential).
 NP_BIND 1885 1893 ATP (By similarity).
 NP_BIND 2098 2121 GTP (Potential).
 NP_BIND 2295 2298 GTP (Potential).
 ACT_SITE 1994 1994 Proton acceptor (By similarity).
 BINDING 1906 1906 ATP (By similarity).  
Keyword
 ATP-binding; Autophagy; Coiled coil; Complete proteome; Cytoplasm; GTP-binding; GTPase activation; Kinase; Leucine-rich repeat; Membrane; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2527 AA 
Protein Sequence
MASGACQGCE EEEEEEALKK LIVRLNNVQE GKQIETLLQL LEDMLVFTYS DRASKLFEDK 60
NFHVPLLIVL DSYMRVASVQ QAGWSLLCKL IEVCPGTLQS LIGPQDIGND WEVLGIHRLI 120
LKMLTVHHAN VNLSIVGLKA LDLLLDSGKL TLLILDEECD IFLLIFDAMH RYSANDEVQK 180
LGCKALHVLF ERVSEEQLTE FVENKDYTIL LSTFGSFRRD KEIVYHVLCC LHSLAVTCSN 240
VEVLMSGNVR CYNLVVEAMK AFPTNENIQE VSCSLFQKLT LGNFFNILVL NEVHVFVVKA 300
VRQYPENAAL QISALSCLAL LTETIFLNQD LEERSETQEQ SEEEDSEKLF WLEPCYKALV 360
RHRKDKHVQE AACWALNNLL MYQNSLHEKI GDEDGQFPAH REVMLSMLMH SSSKDVFQAA 420
AHALSTLLEQ NVNFRKILLA KGVYLNVLEL MQKHAHAPEV AESGCKMLSH LFEGSNPSLD 480
TMAAVVPKIL TVMKAHGTSL SVQLEALRAI LHFVVPGLLE ESREDSQCRP NVLRKQCFRT 540
DIHKLVLVAL NRFIGNPGIQ KCGLKVISSL AHLPDATETL SLQGAVDSVL HTLQMYPDDQ 600
EIQCLGLHLM GCLMTKKNFC IGTGHLLAKI LASTLQRFKD VAEVQTTGLQ TTLSILELSV 660
SFSKLLVHYS FDVVIFHQMS SSVVEQKDEQ FLNLCCKCFA KVAVDDELKN TMLERACDQN 720
NSIMVECLLL LGADANQVKG ATSLIYQVCE KESSPKLVEL LLNGGCREQD VRKALTVSIQ 780
KGDSQVISLL LRKLALDLAN NSICLGGFGI GKIDPSWLGP LFPDKSSNLR KQTNTGSVLA 840
RKVLRYQMRN TLQEGVASGS DGKFSEDALA KFGEWTFIPD SSMDSVFGQS DDLDSEGSES 900
SFLVKRKSNS ISVGEVYRDL ALQRCSPNAQ RHSNSLGPVF DHEDLLRRKR KILSSDESLR 960
SSRLPSHMRQ SDSSSSLASE REHITSLDLS ANELKDIDAL SQKCCLSSHL EHLTKLELHQ 1020
NSLTSFPQQL CETLKCLIHL DLHSNKFTSF PSFVLKMPRI TNLDASRNDI GPTVVLDPAM 1080
KCPSLKQLNL SYNQLSSIPE NLAQVVEKLE QLLLEGNKIS GICSPLSLKE LKILNLSKNH 1140
IPSLPGDFLE ACSKVESFSA RMNFLAAMPA LPSSITSLKL SQNSFTCIPE AIFSLPHLRS 1200
LDMSHNNIEC LPGPAHWKSL NLRELIFSKN QISTLDFSEN PHVWSRVEKL HLSHNKLKEI 1260
PPEIGCLENL TSLDVSYNLE LRSFPNEMGK LSKIWDLPLD GLHLNFDFKH VGCKAKDIIR 1320
FLQQRLKKAV PYNRMKLMIV GNTGSGKTTL LQQLMKMKKP ELGMQGATVG IDVRDWSIQI 1380
RGKRRKDLVL NVWDFAGREE FYSTHPHFMT QRALYLAVYD LSKGQAEVDA MKPWLFNIKA 1440
RASSSPVILV GTHLDVSDEK QRKACISKIT KELLNKRGFP TIRDYHFVNA TEESDALAKL 1500
RKTIINESLN FKIRDQPVVG QLIPDCYVEL EKIILSERKA VPTEFPVINR KHLLQLVNEH 1560
QLQLDENELP HAVHFLNESG VLLHFQDPAL QLSDLYFVEP KWLCKVMAQI LTVKVDGCLK 1620
HPKGIISRRD VEKFLSKKKR FPKNYMMQYF KLLEKFQIAL PIGEEYLLVP SSLSDHRPVI 1680
ELPHCENSEI IIRLYEMPYF PMGFWSRLIN RLLEISPFML SGRERALRPN RMYWRQGIYL 1740
NWSPEAYCLV GSEVLDNRPE SFLKITVPSC RKGCILLGRV VDHIDSLMEE WFPGLLEIDI 1800
CGEGETLLKK WALYSFNDGE EHQKILLDEL MKKAEEGDLL INPDQPRLTI PISQIAPDLI 1860
LADLPRNIML NNDELEFEEA PEFLLGDGSF GSVYRAAYEG EEVAVKIFNK HTSLRLLRQE 1920
LVVLCHLHHP SLISLLAAGI RPRMLVMELA SKGSLDRLLQ QDKASLTRTL QHRIALHVAD 1980
GLRYLHSAMI IYRDLKPHNV LLFTLYPNAA IIAKIADYGI AQYCCRMGIK TSEGTPGFRA 2040
PEVARGNVIY NQQADVYSFG LLLHDIWTTG SRIMEGLRFP NEFDELAIQG KLPDPVKEYG 2100
CAPWPMVEKL ITKCLKENPQ ERPTSAQVFD ILNSAELICL MRHILIPKNI IVECMVATNL 2160
NSKSATLWLG CGNTEKGQLS LFDLNTERYS YEEVADSRIL CLALVHLAAE KESWVVCGTQ 2220
SGALLVINVE EETKRHTLEK MTDSVTCLHC NSLAKQSKQS NFLLVGTADG NLMIFEDKAV 2280
KCKGAAPLKT LHIGDVSTPL MCLSESLNSS ERHITWGGCG TKVFSFSNDF TIQKLIETKT 2340
NQLFSYAAFS DSNIIALAVD TALYIAKKNS PVVEVWDKKT EKLCELIDCV HFLKEVMVKL 2400
NKESKHQLSY SGRVKALCLQ KNTALWIGTG GGHILLLDLS TRRVIRTIHN FCDSVRAMAT 2460
AQLGSLKNVM LVLGYKRKST EGIQEQKEIQ SCLSIWDLNL PHEVQNLEKH IEVRTELADK 2520
MRKTSVE 2527 
Gene Ontology
 GO:0032839; C:dendrite cytoplasm; IEA:Compara.
 GO:0032473; C:external side of mitochondrial outer membrane; IEA:Compara.
 GO:0045121; C:membrane raft; IDA:MGI.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0008021; C:synaptic vesicle; IDA:MGI.
 GO:0005802; C:trans-Golgi network; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005525; F:GTP binding; ISA:MGI.
 GO:0034211; F:GTP-dependent protein kinase activity; ISA:MGI.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:Compara.
 GO:0004708; F:MAP kinase kinase activity; IEA:Compara.
 GO:0000186; P:activation of MAPKK activity; IEA:Compara.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0008340; P:determination of adult lifespan; IEA:Compara.
 GO:0035640; P:exploration behavior; IEA:Compara.
 GO:0048312; P:intracellular distribution of mitochondria; IEA:Compara.
 GO:2000173; P:negative regulation of branching morphogenesis of a nerve; IEA:Compara.
 GO:0061002; P:negative regulation of dendritic spine morphogenesis; IEA:Compara.
 GO:0034260; P:negative regulation of GTPase activity; ISO:MGI.
 GO:0007406; P:negative regulation of neuroblast proliferation; IEA:Compara.
 GO:0014043; P:negative regulation of neuron maturation; IEA:Compara.
 GO:0007528; P:neuromuscular junction development; IEA:Compara.
 GO:0070997; P:neuron death; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IEA:Compara.
 GO:0018107; P:peptidyl-threonine phosphorylation; IEA:Compara.
 GO:0010508; P:positive regulation of autophagy; IMP:BHF-UCL.
 GO:0060161; P:positive regulation of dopamine receptor signaling pathway; IEA:Compara.
 GO:0043068; P:positive regulation of programmed cell death; IEA:Compara.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:BHF-UCL.
 GO:0031398; P:positive regulation of protein ubiquitination; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; ISA:MGI.
 GO:0035564; P:regulation of kidney size; IMP:BHF-UCL.
 GO:0040012; P:regulation of locomotion; IEA:Compara.
 GO:0042391; P:regulation of membrane potential; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0022028; P:tangential migration from the subventricular zone to the olfactory bulb; IEA:Compara. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR011009; Kinase-like_dom.
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR020859; ROC_GTPase.
 IPR008271; Ser/Thr_kinase_AS.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00560; LRR_1
 PF12799; LRR_4
 PF08477; Miro
 PF00069; Pkinase 
SMART
 SM00248; ANK
 SM00320; WD40 
PROSITE
 PS51450; LRR
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS51424; ROC 
PRINTS
 PR00449; RASTRNSFRMNG.