CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003067
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tryptophan synthase beta chain 
Protein Synonyms/Alias
  
Gene Name
 trpB 
Gene Synonyms/Alias
 b1261; JW1253 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
37EAFVSAQKDPEFQAQacetylation[1]
50AQFNDLLKNYAGRPTacetylation[1]
99LGQALLAKRMGKTEIacetylation[1]
137CRIYMGAKDVERQSPacetylation[1]
213RMIGEETKAQILEREacetylation[1]
368MMRENPDKEQLLVVNacetylation[1]
382NLSGRGDKDIFTVHDacetylation[1]
392FTVHDILKARGEI**acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. 
Sequence Annotation
 ACT_SITE 62 62 Nucleophile.
 ACT_SITE 86 86 Proton donor (Probable).
 MOD_RES 87 87 N6-(pyridoxal phosphate)lysine (By  
Keyword
 3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Complete proteome; Direct protein sequencing; Lyase; Pyridoxal phosphate; Reference proteome; Tryptophan biosynthesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 397 AA 
Protein Sequence
MTTLLNPYFG EFGGMYVPQI LMPALRQLEE AFVSAQKDPE FQAQFNDLLK NYAGRPTALT 60
KCQNITAGTN TTLYLKREDL LHGGAHKTNQ VLGQALLAKR MGKTEIIAET GAGQHGVASA 120
LASALLGLKC RIYMGAKDVE RQSPNVFRMR LMGAEVIPVH SGSATLKDAC NEALRDWSGS 180
YETAHYMLGT AAGPHPYPTI VREFQRMIGE ETKAQILERE GRLPDAVIAC VGGGSNAIGM 240
FADFINETNV GLIGVEPGGH GIETGEHGAP LKHGRVGIYF GMKAPMMQTE DGQIEESYSI 300
SAGLDFPSVG PQHAYLNSTG RADYVSITDD EALEAFKTLC LHEGIIPALE SSHALAHALK 360
MMRENPDKEQ LLVVNLSGRG DKDIFTVHDI LKARGEI 397 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0030170; F:pyridoxal phosphate binding; IDA:EcoliWiki.
 GO:0004834; F:tryptophan synthase activity; IDA:EcoCyc. 
Interpro
 IPR001926; Trp_syn_b_sub_like_PLP_eny_SF.
 IPR006653; Trp_synth_b_CS.
 IPR006654; Trp_synth_beta.
 IPR023026; Trp_synth_beta/beta-like. 
Pfam
 PF00291; PALP 
SMART
  
PROSITE
 PS00168; TRP_SYNTHASE_BETA 
PRINTS