CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041285
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exosome complex exonuclease RRP44 
Protein Synonyms/Alias
 KIAA1008, isoform CRA_c 
Gene Name
 DIS3 
Gene Synonyms/Alias
 KIAA1008; hCG_32200 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
423TKSVINSKASLTYAEubiquitination[1]
492DPIDLQTKELRETNSubiquitination[1]
515ANISVAKKIHEEFSEubiquitination[1]
528SEHALLRKHPAPPPSubiquitination[1]
552RSRNLEIKTDTAKSLubiquitination[1, 2, 3]
647PELTDKHKLADICKNubiquitination[1]
653HKLADICKNLNFRHKubiquitination[1, 4]
660KNLNFRHKMAQYAQRubiquitination[5]
744TVFHVFDKVKVKIMLubiquitination[4]
746FHVFDKVKVKIMLDSubiquitination[1]
760SSNLQHQKIRMSLVEacetylation[3, 6]
760SSNLQHQKIRMSLVEubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 796 AA 
Protein Sequence
MSADNQLQVI FITNDRRNKE KAIEEGIPAF TCEEYVKSLT ANPELIDRLA CLSEEGNEIE 60
SGKIIFSEHL PLSKLQQGIK SGTYLQGTFR ASRENYLEAT VWIHGDNEEN KEIILQGLKH 120
LNRAVHEDIV AVELLPKSQW VAPSSVVLHD EGQNEEDVEK EEETERMLKT AVSEKMLKPT 180
GRVVGIIKRN WRPYCGMLSK SDIKESRRHL FTPADKRIPR IRIETRQAST LEGRRIIVAI 240
DGWPRNSRYP NGHFVRNLGD VGEKETETEV LLLEHDVPHQ PFSQAVLSFL PKMPWSITEK 300
DMKNREDLRH LCICSVDPPG CTDIDDALHC RELENGNLEV GVHIADVSHF IRPGNALDQE 360
SARRGTTVYL CEKRIDMVPE LLSSNLCSLK CDVDRLAFSC IWEMNHNAEI LKTKFTKSVI 420
NSKASLTYAE AQLRIDSANM NDDITTSLRG LNKLAKILKK RRIEKGALTL SSPEVRFHMD 480
SETHDPIDLQ TKELRETNSM VEEFMLLANI SVAKKIHEEF SEHALLRKHP APPPSNYEIL 540
VKAARSRNLE IKTDTAKSLA ESLDQAESPT FPYLNTLLRI LATRCMMQAV YFCSGMDNDF 600
HHYGLASPIY THFTSPIRRY ADVIVHRLLA VAIGADCTYP ELTDKHKLAD ICKNLNFRHK 660
MAQYAQRASV AFHTQLFFKS KGIVSEEAYI LFVRKNAIVV LIPKYGLEGT VFFEEKDKPN 720
PQLIYDDEIP SLKIEDTVFH VFDKVKVKIM LDSSNLQHQK IRMSLVEPQI PGISIPTDTS 780
NMDLNGPKKK KMKLGK 796 
Gene Ontology
 GO:0004540; F:ribonuclease activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro. 
Interpro
 IPR001900; RNase_II/R.
 IPR022966; RNase_II/R_CS. 
Pfam
 PF00773; RNB 
SMART
 SM00955; RNB 
PROSITE
 PS01175; RIBONUCLEASE_II 
PRINTS