CPLM-020537

Genbank Nucleotide ID

Heat shock protein 75 kDa, mitochondrial

Protein Synonyms/Alias

HSP 75; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1

Mus musculus (Mouse)

Position	Peptide	Type	References
89	AVRGSVSKHEFQAET	acetylation	[1, 2]
111	ARSLYSEKEVFIREL	acetylation	[3]
152	IHLQTDAKKGTITIQ	acetylation	[3]
264	IHLKSDCKDFASESR	acetylation	[2, 3, 4]
326	YIAQAYDKPRFTLHY	acetylation	[2, 3, 5]
326	YIAQAYDKPRFTLHY	succinylation	[5]
334	PRFTLHYKTDAPLNI	acetylation	[2, 3, 4]
334	PRFTLHYKTDAPLNI	ubiquitination	[6]
351	IFYVPEMKPSMFDVS	acetylation	[4]
426	VLQQRLIKFFIDQSK	acetylation	[2, 3, 4, 5, 7]
426	VLQQRLIKFFIDQSK	succinylation	[5]
433	KFFIDQSKKDAEKYA	acetylation	[2, 3]
438	QSKKDAEKYAKFFED	acetylation	[3]
579	ADERLSEKETEDLMA	acetylation	[4]
654	NPRHTLIKKLCQLRE	acetylation	[3]

[1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[7] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]

Functional Description

Chaperone that expresses an ATPase activity (By similarity).

BINDING 121 121 ATP (By similarity).
BINDING 160 160 ATP (By similarity).
BINDING 173 173 ATP (By similarity).
BINDING 207 207 ATP; via amide nitrogen (By similarity).
BINDING 404 404 ATP (By similarity).
MOD_RES 89 89 N6-acetyllysine.
MOD_RES 172 172 Phosphoserine (By similarity).
MOD_RES 176 176 Phosphothreonine (By similarity).
MOD_RES 334 334 N6-acetyllysine (By similarity).
MOD_RES 403 403 Phosphoserine (By similarity).
MOD_RES 426 426 N6-acetyllysine (By similarity).
MOD_RES 468 468 N6-acetyllysine (By similarity).
MOD_RES 496 496 Phosphothreonine (By similarity).

Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0034599; P:cellular response to oxidative stress; IEA:Compara.
GO:0006457; P:protein folding; IEA:InterPro.

IPR003594; HATPase_ATP-bd.
IPR001404; Hsp90.
IPR020575; Hsp90_N.
IPR020568; Ribosomal_S5_D2-typ_fold.

PR00775; HEATSHOCK90.