CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022582
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein WRAP73 
Protein Synonyms/Alias
 WD repeat-containing protein 8; WD repeat-containing protein antisense to TP73 gene 
Gene Name
 WRAP73 
Gene Synonyms/Alias
 WDR8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130TKSVSYIKYPKACLQubiquitination[1]
246AVGSYDGKVRILNHVubiquitination[1, 2]
276DPKIVVYKEAEKSPQubiquitination[1, 2, 3]
280VVYKEAEKSPQLGLGubiquitination[3]
307PLPSSESKYEIASVPubiquitination[1, 2, 3]
321PVSLQTLKPVTDRANubiquitination[1, 2, 3]
330VTDRANPKIGIGMLAubiquitination[2, 3, 4, 5]
363VWVWDIQKLRLFAVLubiquitination[1, 2, 3, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 REPEAT 46 86 WD 1.
 REPEAT 89 129 WD 2.
 REPEAT 176 210 WD 3.
 REPEAT 221 260 WD 4.
 REPEAT 328 369 WD 5.
 REPEAT 371 410 WD 6.  
Keyword
 Complete proteome; Cytoplasm; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 460 AA 
Protein Sequence
MNFSEVFKLS SLLCKFSPDG KYLASCVQYR LVVRDVNTLQ ILQLYTCLDQ IQHIEWSADS 60
LFILCAMYKR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS 120
LCTKSVSYIK YPKACLQGIT FTRDGRYMAL AERRDCKDYV SIFVCSDWQL LRHFDTDTQD 180
LTGIEWAPNG CVLAVWDTCL EYKILLYSLD GRLLSTYSAY EWSLGIKSVA WSPSSQFLAV 240
GSYDGKVRIL NHVTWKMITE FGHPAAINDP KIVVYKEAEK SPQLGLGCLS FPPPRAGAGP 300
LPSSESKYEI ASVPVSLQTL KPVTDRANPK IGIGMLAFSP DSYFLATRND NIPNAVWVWD 360
IQKLRLFAVL EQLSPVRAFQ WDPQQPRLAI CTGGSRLYLW SPAGCMSVQV PGEGDFAVLS 420
LCWHLSGDSM ALLSKDHFCL CFLETEAVVG TACRQLGGHT 460 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS