CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032295
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PDE3B protein 
Protein Synonyms/Alias
 cGMP-inhibited 3',5'-cyclic phosphodiesterase B 
Gene Name
 PDE3B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33SLRNGYVKSCVSPLRubiquitination[1, 2]
285LSSAAEEKVPVIRPRubiquitination[1, 2]
312ASYYGSCKIFRRPSLubiquitination[1, 2]
339WDLKQWYKPHYQNSGubiquitination[1, 2, 3]
495DTADFLNKPSVILQRubiquitination[1, 2, 4]
534SCGHQMLKYVSTSESubiquitination[1]
550GTDCCSGKSGEEENIubiquitination[1, 2]
560EEENIFSKESFKLMEubiquitination[1, 2, 4]
576QQEEETEKKDSRKLFubiquitination[1, 2]
577QEEETEKKDSRKLFQubiquitination[1]
892ADINGPAKVRDLHLKubiquitination[1, 2, 4]
1030EIVEEEEKCKADGNKubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1061 AA 
Protein Sequence
MRRDERDAKA MRSLQPPDGA GSPPESLRNG YVKSCVSPLR QDPPRGFFFH LCRFCNVELR 60
PPPASPQQPR RCSPFCRARL SLGALAAFVL ALLLGAEPES WAAGAAWLRT LLSVCSHSLS 120
PLFSIACAFF FLTCFLTRTK RGPGPGRSCG SWWLLALPAC CYLGDFLVWQ WWSWPWGDGD 180
AGSAAPHTPP EAAAGRLLLV LSCVGLLLTL AHPLRLRHCV LVLLLASFVW WVSFTSLGSL 240
PSALRPLLSG LVGGAGCLLA LGLDHFFQIR EAPLHPRLSS AAEEKVPVIR PRRRSSCVSL 300
GETAASYYGS CKIFRRPSLP CISREQMILW DWDLKQWYKP HYQNSGGGNG VDLSVLNEAR 360
NMVSDLLTDP SLPPQGLNRN SLPTPQLRRS SGTSGLLPVE QSSRWDRNNG KRPHQEFGIS 420
SQGCYLNGPF NSNLLTIPKQ RSSSVSLTHH VGLRRAGVLS SLSPVNSSNH GPVSTGSLTN 480
RSPIEFPDTA DFLNKPSVIL QRSLGNAPNT PDFYQQLRNS DSNLCNSCGH QMLKYVSTSE 540
SDGTDCCSGK SGEEENIFSK ESFKLMETQQ EEETEKKDSR KLFQEGDKWL TEEAQSEQQT 600
NIEQEVSLDL ILVEEYDSLI EKMSNWNFPI FELVEKMGEK SGRILSQVMY TLFQDTGLLE 660
IFKIPTQQFM NYFRALENGY RDIPYHNRIH ATDVLHAVWY LTTRPVPGLQ QIHNGCGTGN 720
ETDSDGRINH GRIAYISSKS CSNPDESYGC LSSNIPALEL MALYVAAAMH DYDHPGRTNA 780
FLVATNAPQA VLYNDRSVLE NHHAASAWNL YLSRPEYNFL LHLDHVEFKR FRFLVIEAIL 840
ATDLKKHFDF LAEFNAKAND VNSNGIEWSN ENDRLLVCQV CIKLADINGP AKVRDLHLKW 900
TEGIVNEFYE QGDEEANLGL PISPFMDRSS PQLAKLQESF ITHIVGPLCN SYDAAGLLPG 960
QWLEAEEDND TESGDDEDGE ELDTEDEEME NNLNPKPPRR KSRRRIFCQL MHHLTENHKI 1020
WKEIVEEEEK CKADGNKLQV ENSSLPQADE IQVIEEADEE E 1061 
Gene Ontology
 GO:0004114; F:3',5'-cyclic-nucleotide phosphodiesterase activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008152; P:metabolic process; IEA:GOC.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR003607; HD/PDEase_dom.
 IPR002073; PDEase_catalytic_dom.
 IPR023174; PDEase_CS. 
Pfam
 PF00233; PDEase_I 
SMART
 SM00471; HDc 
PROSITE
 PS00126; PDEASE_I 
PRINTS