CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005913
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GTP cyclohydrolase 1 
Protein Synonyms/Alias
 GTP cyclohydrolase I; GTP-CH-I 
Gene Name
 GCH1 
Gene Synonyms/Alias
 DYT5; GCH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
54AQPADGWKGERPRSEubiquitination[1]
93PQRQGLLKTPWRAASubiquitination[1, 2]
160HIGYLPNKQVLGLSKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. 
Sequence Annotation
 METAL 141 141 Zinc.
 METAL 144 144 Zinc.
 METAL 212 212 Zinc.
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 81 81 Phosphoserine.  
Keyword
 3D-structure; Allosteric enzyme; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Dystonia; GTP-binding; Hydrolase; Metal-binding; Nucleotide-binding; Nucleus; Parkinsonism; Phenylketonuria; Phosphoprotein; Polymorphism; Reference proteome; Tetrahydrobiopterin biosynthesis; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 250 AA 
Protein Sequence
MEKGPVRAPA EKPRGARCSN GFPERDPPRP GPSRPAEKPP RPEAKSAQPA DGWKGERPRS 60
EEDNELNLPN LAAAYSSILS SLGENPQRQG LLKTPWRAAS AMQFFTKGYQ ETISDVLNDA 120
IFDEDHDEMV IVKDIDMFSM CEHHLVPFVG KVHIGYLPNK QVLGLSKLAR IVEIYSRRLQ 180
VQERLTKQIA VAITEALRPA GVGVVVEATH MCMVMRGVQK MNSKTVTSTM LGVFREDPKT 240
REEFLTLIRS 250 
Gene Ontology
 GO:0031410; C:cytoplasmic vesicle; IDA:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; IEA:Compara.
 GO:0050662; F:coenzyme binding; IEA:Compara.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003934; F:GTP cyclohydrolase I activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0035998; P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0042416; P:dopamine biosynthetic process; IDA:UniProtKB.
 GO:0006184; P:GTP catabolic process; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0045776; P:negative regulation of blood pressure; IEA:Compara.
 GO:0050884; P:neuromuscular process controlling posture; IMP:MGI.
 GO:0006809; P:nitric oxide biosynthetic process; NAS:UniProtKB.
 GO:0051000; P:positive regulation of nitric-oxide synthase activity; IDA:UniProtKB.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
 GO:0008217; P:regulation of blood pressure; IMP:UniProtKB.
 GO:0014916; P:regulation of lung blood pressure; IEA:Compara.
 GO:0034341; P:response to interferon-gamma; IDA:UniProtKB.
 GO:0032496; P:response to lipopolysaccharide; IDA:UniProtKB.
 GO:0048265; P:response to pain; ISS:UniProtKB.
 GO:0034612; P:response to tumor necrosis factor; IDA:UniProtKB.
 GO:0006729; P:tetrahydrobiopterin biosynthetic process; IDA:UniProtKB.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:InterPro.
 GO:0042311; P:vasodilation; IEA:Compara. 
Interpro
 IPR001474; GTP_CycHdrlase_I.
 IPR020602; GTP_CycHdrlase_I/CN_OxRdtase.
 IPR018234; GTP_CycHdrlase_I_CS. 
Pfam
 PF01227; GTP_cyclohydroI 
SMART
  
PROSITE
 PS00859; GTP_CYCLOHYDROL_1_1
 PS00860; GTP_CYCLOHYDROL_1_2 
PRINTS