UniProt Accession

 API5_HUMAN; Q9BZZ5; B4DGR0; B4DRJ2; D3DR21; O15441; Q9Y4J7 

Genbank Protein ID

 U83857; Y15906; AF229253; AF229254; AY311389; BT007093; AY265973; AK294724; AK299288; AC087276; CH471064; CH471064; BC017709; W40304 

Genbank Nucleotide ID

 AAB86528.1; CAA75867.1; AAK00737.1; AAK00738.1; AAQ76714.1; AAP35756.1; AAO89077.1; BAG57871.1; BAG61304.1; EAW68098.1; EAW68099.1; AAH17709.1 

Protein Name

 Apoptosis inhibitor 5 

Protein Synonyms/Alias

 API-5; Antiapoptosis clone 11 protein; AAC-11; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL 

Gene Name

 API5 

Gene Synonyms/Alias

 MIG8 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
36	QVILDGVKGGTKEKR	ubiquitination	[1]
51	LAAQFIPKFFKHFPE	ubiquitination	[1]
84	SIRRQAIKELPQFAT	ubiquitination	[1]
160	KFLSTKLKTLPDEVL	ubiquitination	[2]
487	IYNPPSGKYSSNLGN	ubiquitination	[1, 2, 3]

Reference

 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. 

Sequence Annotation

 REGION 2 360 ARM-like and Heat-like helical repeats.
 REGION 370 391 Leucine-zipper.
 MOTIF 454 475 Nuclear localization signal.
 MOD_RES 251 251 N6-acetyllysine.
 MOD_RES 462 462 Phosphoserine.
 MOD_RES 464 464 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 524 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; NAS:UniProtKB.
 GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB. 

Interpro

 IPR008383; API5.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold. 

Pfam

 PF05918; API5 

SMART

  

PROSITE

  

PRINTS