CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001194
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 24 
Protein Synonyms/Alias
 Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; Mediator complex subunit 24; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; hTRAP100; Vitamin D3 receptor-interacting protein complex 100 kDa component; DRIP100 
Gene Name
 MED24 
Gene Synonyms/Alias
 ARC100; CRSP4; DRIP100; KIAA0130; THRAP4; TRAP100 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
156GTPAAGEKQLAMCLQubiquitination[1]
167MCLQRLEKTLSSTKNubiquitination[1]
350KLTPLLDKADQRCNCubiquitination[1]
460AAAAATGKLKSFARKubiquitination[1]
462AAATGKLKSFARKFIubiquitination[1]
484YGSEESTKPASVRALubiquitination[1]
638LGLDEREKSLQMIRQubiquitination[1]
717VLTDIFAKVLEKGWVubiquitination[2, 3]
721IFAKVLEKGWVDSRSubiquitination[1, 2, 3]
856LDDVQPSKLMRLLSSubiquitination[1]
965VSAMSSPKVVLAITDubiquitination[1]
984LGRQVAAKAIAAL**ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
Sequence Annotation
 MOTIF 128 132 LXXLL motif 1.
 MOTIF 344 348 LXXLL motif 2.
 MOTIF 448 452 LXXLL motif 3.
 MOTIF 557 561 LXXLL motif 4.
 MOTIF 788 792 LXXLL motif 5.
 MOTIF 857 861 LXXLL motif 6.
 MOD_RES 862 862 Phosphoserine.
 MOD_RES 873 873 Phosphoserine.  
Keyword
 Activator; Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 989 AA 
Protein Sequence
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA MIGPSPNPLI 60
LSYLKYAISS QMVSYSSVLT AISKFDDFSR DLCVQALLDI MDMFCDRLSC HGKAEECIGL 120
CRALLSALHW LLRCTAASAE RLREGLEAGT PAAGEKQLAM CLQRLEKTLS STKNRALLHI 180
AKLEEASSWT AIEHSLLKLG EILANLSNPQ LRSQAEQCGT LIRSIPTMLS VHAEQMHKTG 240
FPTVHAVILL EGTMNLTGET QSLVEQLTMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT 300
EELKWTAFTF LKIPQVLVKL KKYSHGDKDF TEDVNCAFEF LLKLTPLLDK ADQRCNCDCT 360
NFLLQECGKQ GLLSEASVNN LMAKRKADRE HAPQQKSGEN ANIQPNIQLI LRAEPTVTNI 420
LKTMDADHSK SPEGLLGVLG HMLSGKSLDL LLAAAAATGK LKSFARKFIN LNEFTTYGSE 480
ESTKPASVRA LLFDISFLML CHVAQTYGSE VILSESRTGA EVPFFETWMQ TCMPEEGKIL 540
NPDHPCFRPD STKVESLVAL LNNSSEMKLV QMKWHEACLS ISAAILEILN AWENGVLAFE 600
SIQKITDNIK GKVCSLAVCA VAWLVAHVRM LGLDEREKSL QMIRQLAGPL FSENTLQFYN 660
ERVVIMNSIL ERMCADVLQQ TATQIKFPST GVDTMPYWNL LPPKRPIKEV LTDIFAKVLE 720
KGWVDSRSIH IFDTLLHMGG VYWFCNNLIK ELLKETRKEH TLRAVELLYS IFCLDMQQVT 780
LVLLGHILPG LLTDSSKWHS LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI 840
EDYISLFPLD DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLHT VNMRDPLNRV 900
LANLFLLISS ILGSRTAGPH TQFVQWFMEE CVDCLEQGGR GSVLQFMPFT TVSELVKVSA 960
MSSPKVVLAI TDLSLPLGRQ VAAKAIAAL 989 
Gene Ontology
 GO:0016592; C:mediator complex; NAS:UniProtKB.
 GO:0004402; F:histone acetyltransferase activity; IEA:Compara.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; NAS:UniProtKB.
 GO:0004872; F:receptor activity; IDA:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IDA:UniProtKB.
 GO:0046966; F:thyroid hormone receptor binding; IDA:UniProtKB.
 GO:0042809; F:vitamin D receptor binding; NAS:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:UniProtKB. 
Interpro
 IPR021429; Mediator_Med24_N. 
Pfam
 PF11277; Med24_N 
SMART
  
PROSITE
  
PRINTS