CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012911
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Oxysterol-binding protein 1 
Protein Synonyms/Alias
  
Gene Name
 Osbp 
Gene Synonyms/Alias
 Kiaa4220 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
215TLRTLSSKVEDLSTCubiquitination[1]
228TCNDLIAKHGTALQRacetylation[2]
228TCNDLIAKHGTALQRubiquitination[1]
290THSKKWQKSLQYERDubiquitination[1]
311ETLEQLAKQHNHLERubiquitination[1]
427IMKNCIGKELSKIPMubiquitination[1]
431CIGKELSKIPMPVNFubiquitination[1]
491TTVFRTSKPFNPLLGacetylation[2]
602EIDIVNHKTGDKCNLubiquitination[1]
754KREAEAAKATEDGTPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25- hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability (By similarity). 
Sequence Annotation
 DOMAIN 86 179 PH.
 REGION 404 455 Sterol binding (By similarity).
 MOD_RES 101 101 N6-acetyllysine (By similarity).
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 191 191 Phosphoserine.
 MOD_RES 196 196 Phosphoserine.
 MOD_RES 236 236 Phosphoserine.
 MOD_RES 349 349 Phosphoserine.
 MOD_RES 375 375 Phosphothreonine.
 MOD_RES 377 377 Phosphoserine.
 MOD_RES 380 380 Phosphoserine.
 MOD_RES 387 387 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Golgi apparatus; Lipid transport; Lipid-binding; Membrane; Phosphoprotein; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 805 AA 
Protein Sequence
MAATELRGVV GPGPAAIAAP GGGGAGPPAV GGGGGRGDAG PGPGVAAATA ATAGGPGPGA 60
GGVAAGGPGS APPAAGSGGS GAGGSGSARE GWLFKWTNYI KGYQRRWFVL SNGLLSYYRS 120
KAEMRHTCRG TINLATANIT VEDSCNFIIS NGGAQTYHLK ASSEVERQRW VTALELAKAK 180
AVKMLAESDD SGDEESVSQT DKTELQSTLR TLSSKVEDLS TCNDLIAKHG TALQRSLSEL 240
ESLKLPAESN EKIKQVNERA TLFRITSNAM INACRDFLML AQTHSKKWQK SLQYERDQRI 300
RLEETLEQLA KQHNHLERAF RGATVLPANP PGSAGSGKDQ CCSGKGDMSD EDDENEFFDA 360
PEIITMPENL GHKRTGSNIS GASSDVSLDE QYKHQLEETK KEKRTRIPYK PNYSLNLWSI 420
MKNCIGKELS KIPMPVNFNE PLSMLQRLTE DLEYHELLDR AAKCENSLEQ LCYVAAFTVS 480
SYSTTVFRTS KPFNPLLGET FELDRLEENG YRSICEQVSH HPPAAAHHAE SKNGWTLRQE 540
IKITSKFRGK YLSIMPLGTI HCIFHSTGHH YTWKKVTTTV HNIIVGKLWI DQSGEIDIVN 600
HKTGDKCNLK FVPYSYFSRD VARKVTGEVT DPSGKVHFAL LGTWDEKMDC FKVQAASGEN 660
GGDARQRGHE AEDSRVMLWK RNPLPKNAEN MYYFSELALT LNAWEGGTAP TDSRLRPDQR 720
LMENGRWDEA NAEKQRLEEK QRLSRKKREA EAAKATEDGT PHDPYKALWF ERKKDPVTRE 780
LTHIYSGEYW ECKEKQDWGS CPDIF 805 
Gene Ontology
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0006869; P:lipid transport; IEA:UniProtKB-KW. 
Interpro
 IPR000648; Oxysterol-bd.
 IPR018494; Oxysterol-bd_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF01237; Oxysterol_BP
 PF00169; PH 
SMART
 SM00233; PH 
PROSITE
 PS01013; OSBP
 PS50003; PH_DOMAIN 
PRINTS