CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012310
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Guanine nucleotide-binding protein subunit alpha-13 
Protein Synonyms/Alias
 G alpha-13; G-protein subunit alpha-13 
Gene Name
 GNA13 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36RKSKEIDKCLSREKTubiquitination[1, 2]
183YFLDNLDKLGEPDYIubiquitination[3]
214HEYDFEIKNVPFKMVubiquitination[1, 3, 4, 5, 6]
219EIKNVPFKMVDVGGQubiquitination[1, 5]
363RLVFRDVKDTILHDNubiquitination[6]
372TILHDNLKQLMLQ**ubiquitination[1, 3, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. 
Sequence Annotation
 NP_BIND 55 62 GTP (By similarity).
 NP_BIND 197 203 GTP (By similarity).
 NP_BIND 222 226 GTP (By similarity).
 NP_BIND 291 294 GTP (By similarity).
 METAL 62 62 Magnesium (By similarity).
 METAL 203 203 Magnesium (By similarity).
 BINDING 349 349 GTP; via amide nitrogen (By similarity).
 MOD_RES 203 203 Phosphothreonine; by PKA.
 LIPID 14 14 S-palmitoyl cysteine.
 LIPID 18 18 S-palmitoyl cysteine.  
Keyword
 Complete proteome; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Palmitate; Phosphoprotein; Polymorphism; Reference proteome; Transducer. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 377 AA 
Protein Sequence
MADFLPSRSV LSVCFPGCLL TSGEAEQQRK SKEIDKCLSR EKTYVKRLVK ILLLGAGESG 60
KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV DAREKLHIPW GDNSNQQHGD 120
KMMSFDTRAP MAAQGMVETR VFLQYLPAIR ALWADSGIQN AYDRRREFQL GESVKYFLDN 180
LDKLGEPDYI PSQQDILLAR RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV 240
TSILFLVSSS EFDQVLMEDR LTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV 300
QIVSIKDYFL EFEGDPHCLR DVQKFLVECF RNKRRDQQQK PLYHHFTTAI NTENIRLVFR 360
DVKDTILHDN LKQLMLQ 377 
Gene Ontology
 GO:0031526; C:brush border membrane; IBA:RefGenome.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0031752; F:D5 dopamine receptor binding; IBA:RefGenome.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0031702; F:type 1 angiotensin receptor binding; IBA:RefGenome.
 GO:0031584; P:activation of phospholipase D activity; IBA:RefGenome.
 GO:0007189; P:adenylate cyclase-activating G-protein coupled receptor signaling pathway; IBA:RefGenome.
 GO:0030154; P:cell differentiation; IEA:Compara.
 GO:0006928; P:cellular component movement; TAS:ProtInc.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0007243; P:intracellular protein kinase cascade; IEA:Compara.
 GO:0001569; P:patterning of blood vessels; IEA:Compara.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0030334; P:regulation of cell migration; IEA:Compara.
 GO:0008360; P:regulation of cell shape; IEA:Compara.
 GO:0007266; P:Rho protein signal transduction; IBA:RefGenome. 
Interpro
 IPR000469; Gprotein_alpha_12.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00503; G-alpha 
SMART
 SM00275; G_alpha 
PROSITE
  
PRINTS
 PR00318; GPROTEINA.
 PR00440; GPROTEINA12.