CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011834
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome regulatory subunit RPN6 
Protein Synonyms/Alias
 Proteasome non-ATPase subunit 4 
Gene Name
 RPN6 
Gene Synonyms/Alias
 NAS4; YDL097C; D2381 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
49AGASVDDKRRNEQETubiquitination[1]
98SKTVKVLKTLIEKFEacetylation[2]
376DTQQVEGKLSQMILDubiquitination[1]
429VVDQLFEKASVLY**ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly. 
Sequence Annotation
 DOMAIN 229 401 PCI.
 MOD_RES 2 2 N-acetylserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Proteasome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 434 AA 
Protein Sequence
MSLPGSKLEE ARRLVNEKQY NEAEQVYLSL LDKDSSQSSA AAGASVDDKR RNEQETSILE 60
LGQLYVTMGA KDKLREFIPH STEYMMQFAK SKTVKVLKTL IEKFEQVPDS LDDQIFVCEK 120
SIEFAKREKR VFLKHSLSIK LATLHYQKKQ YKDSLALIND LLREFKKLDD KPSLVDVHLL 180
ESKVYHKLRN LAKSKASLTA ARTAANSIYC PTQTVAELDL MSGILHCEDK DYKTAFSYFF 240
ESFESYHNLT THNSYEKACQ VLKYMLLSKI MLNLIDDVKN ILNAKYTKET YQSRGIDAMK 300
AVAEAYNNRS LLDFNTALKQ YEKELMGDEL TRSHFNALYD TLLESNLCKI IEPFECVEIS 360
HISKIIGLDT QQVEGKLSQM ILDKIFYGVL DQGNGWLYVY ETPNQDATYD SALELVGQLN 420
KVVDQLFEKA SVLY 434 
Gene Ontology
 GO:0008541; C:proteasome regulatory particle, lid subcomplex; IDA:UniProtKB.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0005198; F:structural molecule activity; IMP:SGD.
 GO:0043248; P:proteasome assembly; IMP:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:UniProtKB. 
Interpro
 IPR013143; PAM.
 IPR000717; PCI_dom.
 IPR011990; TPR-like_helical.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF01399; PCI 
SMART
 SM00753; PAM
 SM00088; PINT 
PROSITE
  
PRINTS