UniProt Accession

 PIN3_YEAST; Q06449; D6W4F0 

Genbank Protein ID

 U28371; AY692989; BK006949 

Genbank Nucleotide ID

 AAB68051.1; AAT93008.1; DAA11566.1 

Protein Name

 [PSI+] inducibility protein 3 

Protein Synonyms/Alias

 LAS seventeen-binding protein 2; LAS17-binding protein 2 

Gene Name

 PIN3 

Gene Synonyms/Alias

 LSB2; YPR154W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
36	DVYDQINKSLPAKWD	ubiquitination	[1]
41	INKSLPAKWDPANAP	ubiquitination	[1]
76	QDGDLGLKPGDKVQL	ubiquitination	[1]
80	LGLKPGDKVQLLEKL	ubiquitination	[1, 2, 3, 4, 5]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [5] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Overproduction promotes the de novo induction of the [PSI+] prion form of SUP35. The prion-inducing effect depends on the association with the actin cytoskeleton. Also implicated in prion maintenance during heat stress. 

Sequence Annotation

 DOMAIN 54 113 SH3.
 MOTIF 124 127 PY motif.
 MOD_RES 52 52 Phosphoserine.
 MOD_RES 55 55 Phosphoserine.
 CROSSLNK 80 80 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Cytoskeleton; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; SH3 domain; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0034316; P:negative regulation of Arp2/3 complex-mediated actin nucleation; IDA:SGD. 

Interpro

 IPR000108; p67phox.
 IPR001452; SH3_domain. 

Pfam

 PF00018; SH3_1 

SMART

 SM00326; SH3 

PROSITE

 PS50002; SH3 

PRINTS

 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.