UniProt Accession

 MPPA_MOUSE; Q9DC61; Q3TF19 

Genbank Protein ID

 AK004549; AK147110; AK169325; AK169342; BC010810 

Genbank Nucleotide ID

 BAB23363.1; BAE27682.1; BAE41079.1; BAE41094.1; AAH10810.1 

Protein Name

 Mitochondrial-processing peptidase subunit alpha 

Protein Synonyms/Alias

 Alpha-MPP; P-55 

Gene Name

 Pmpca 

Gene Synonyms/Alias

 Inpp5e 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
298	QYTGGIIKVERDMSN	acetylation	[1]
459	VLATHSRKLPHELCT	acetylation	[2]
472	CTLIRNVKPEDIKRV	acetylation	[1]
477	NVKPEDIKRVASKML	acetylation	[1, 3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Cleaves presequences (transit peptides) from mitochondrial protein precursors (By similarity). 

Sequence Annotation

 MOD_RES 298 298 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Metalloprotease; Mitochondrion; Protease; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 524 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR011249; Metalloenz_LuxS/M16.
 IPR011237; Pept_M16_dom.
 IPR011765; Pept_M16_N.
 IPR001431; Pept_M16_Zn_BS.
 IPR007863; Peptidase_M16_C. 

Pfam

 PF00675; Peptidase_M16
 PF05193; Peptidase_M16_C 

SMART

  

PROSITE

 PS00143; INSULINASE 

PRINTS