UniProt Accession

 MODE_ECOLI; P0A9G8; P46930 

Genbank Protein ID

 U07867; U27192; U34275; U00096; AP009048 

Genbank Nucleotide ID

 AAB06892.1; AAB60175.1; AAA77051.1; AAC73848.1; BAA35425.1 

Protein Name

 Transcriptional regulator ModE 

Protein Synonyms/Alias

  

Gene Name

 modE 

Gene Synonyms/Alias

 modR; b0761; JW0744 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
14	LTLKLQQKLFADPRR	acetylation	[1]
39	GSISQGAKDAGISYK	acetylation	[1]
155	DVLLADGKTRLKVAI	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 The ModE-Mo complex acts as a repressor of the modABC operon, involved in the transport of molybdate. Upon binding molybdate, the conformation of the protein changes, promoting dimerization of ModE-Mo. The protein dimer is then competent to bind a DNA region, upstream of the modABC operon, which contains an 8-base inverted repeat 5'-TAACGTTA-3' flanked by two CAT boxes. Acts also as an enhancer of the expression of genes coding for molybdoenzymes, both directly and indirectly. ModE also interacts with tungstate. 

Sequence Annotation

 DNA_BIND 33 79 H-T-H motif.
 REGION 1 121 I.
 REGION 122 183 IIA (MOP1A).
 REGION 125 133 Required for dimer formation and
 REGION 184 255 IIB (MOP2).
 REGION 256 262 IIA (MOP1B).  

Keyword

 3D-structure; Activator; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Molybdenum; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 262 AA 

Protein Sequence

Gene Ontology

 GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0016820; F:hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; IEA:InterPro.
 GO:0015098; F:molybdate ion transmembrane transporter activity; IEA:InterPro.
 GO:0030151; F:molybdenum ion binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004606; Mo-pterin-bd.
 IPR008995; Mo/tungstate-bd_C_term_dom.
 IPR003725; ModE-bd_N.
 IPR016462; Molybdate-dep_T_reg_ModE.
 IPR005116; Transp-assoc_OB_typ1.
 IPR000847; Tscrpt_reg_HTH_LysR.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00126; HTH_1
 PF03459; TOBE 

SMART

  

PROSITE

  

PRINTS