CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018144
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin light chain kinase 2, skeletal/cardiac muscle 
Protein Synonyms/Alias
 MLCK2 
Gene Name
 Mylk2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
312KEALGGGKFGAVCTCubiquitination[1]
540FVSNLLTKDQSARMSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N- terminus of a myosin light chain (By similarity). 
Sequence Annotation
 DOMAIN 302 557 Protein kinase.
 NP_BIND 308 316 ATP (By similarity).
 REGION 591 603 Calmodulin-binding (By similarity).
 ACT_SITE 423 423 Proton acceptor (By similarity).
 BINDING 331 331 ATP (By similarity).  
Keyword
 ATP-binding; Calmodulin-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 613 AA 
Protein Sequence
MTTENGAVEL GSQSLSTEQT PKAAAGDGPS ASEKEPSAPA TEKDLSPPNA KKDPGAPDPK 60
NNPDPPSLKK DPAKAPGPEK KGDPVPASAS SQGPSGEGDG GGGPAEGSEG PPAALPLPTA 120
TAEASIQKLD PTQAPSGNQG SGEAKAGKKA AECREAGRRG SPAFLHSPSC PAIISCSEKT 180
LAVKPLSETT DLVFTGVSVT PDPQDPGPVK AGGTNALAEK KKEEAEKASG QAGQAKVQGD 240
TPQRIGFQAV PSERVEVGQA LCLTAREEDC FQILDDCPPP PAPFPHRIVE LRTGNVNSEF 300
SMNSKEALGG GKFGAVCTCT ERATGLKLAA KVIKKQTPKD KEMVLLEIEV MNQLNHRNLI 360
QLYAAIETSH EIILFMEYIE GGELFERIVD EDYHLTEVDT MVFVRQICDG ILFMHKMRVL 420
HLDLKPENIL CVNTTGHLVK IIDFGLARRY NPNEKLKVNF GTPEFLSPEV VNYDQISDKT 480
DMWSLGVITY MLLSGLSPFL GDDDTETLNN VLSANWYFDE ETFEAVSDEA KDFVSNLLTK 540
DQSARMSAEQ CLAHPWLNNL AEKAKRCNRR LKSQILLKKY LMKRRWKKNF IAVSAANRFK 600
KISSSGALMA LGV 613 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004687; F:myosin light chain kinase activity; IEA:EC.
 GO:0055007; P:cardiac muscle cell differentiation; NAS:UniProtKB.
 GO:0055008; P:cardiac muscle tissue morphogenesis; IEA:Compara.
 GO:0007274; P:neuromuscular synaptic transmission; IMP:MGI.
 GO:0018107; P:peptidyl-threonine phosphorylation; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0032971; P:regulation of muscle filament sliding; IEA:Compara.
 GO:0014816; P:satellite cell differentiation; IMP:UniProtKB.
 GO:0035914; P:skeletal muscle cell differentiation; NAS:UniProtKB.
 GO:0006941; P:striated muscle contraction; IEA:Compara. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS