CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000739
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial import inner membrane translocase subunit TIM44 
Protein Synonyms/Alias
  
Gene Name
 TIMM44 
Gene Synonyms/Alias
 MIMT44; TIM44 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
217RTEFAGDKFKEEKVFubiquitination[1, 2]
222GDKFKEEKVFEPNEEubiquitination[1, 2]
240VVLHKDSKWYQQWKDubiquitination[1, 2]
246SKWYQQWKDFKENNVubiquitination[1, 2]
264RFFEMKMKYDESDNAubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source. 
Sequence Annotation
 NP_BIND 166 173 ATP (Potential).
 MOD_RES 180 180 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transit peptide; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS YSSGNRKGFL 60
SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA RRKYKTIESE TVRTSEVLRK 120
KLGELTGTVK ESLHEVSKSD LGRKIKEGVE EAAKTAKQSA ESVSKGGEKL GRTAAFRALS 180
QGVESVKKEI DDSVLGQTGP YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK 240
WYQQWKDFKE NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL 300
TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ LAHPIQQAKA 360
LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV VRNPKGEVVE GDPDKVLRML 420
YVWALCRDQD ELNPYAAWRL LDISASSTEQ IL 452 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:BHF-UCL.
 GO:0005759; C:mitochondrial matrix; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015450; F:P-P-bond-hydrolysis-driven protein transmembrane transporter activity; IEA:InterPro.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0006626; P:protein targeting to mitochondrion; TAS:Reactome. 
Interpro
 IPR017303; Tim44.
 IPR007379; Tim44-like_dom. 
Pfam
 PF04280; Tim44 
SMART
 SM00978; Tim44 
PROSITE
  
PRINTS