CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013629
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Meckelin 
Protein Synonyms/Alias
 Meckel syndrome type 3 protein; Transmembrane protein 67 
Gene Name
 TMEM67 
Gene Synonyms/Alias
 MKS3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
98GGPAIICKKCPENMKubiquitination[1]
99GPAIICKKCPENMKGubiquitination[1]
435NQDSNSGKWLLTRRIubiquitination[1]
650RPKGKVLKAVEGEGGubiquitination[1, 2, 3]
810EEMNMNLKREAENLCubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology. Essential for endoplasmic reticulum-associated degradation (ERAD) of surfactant protein C (SFTPC). 
Sequence Annotation
 CARBOHYD 242 242 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Bardet-Biedl syndrome; Cell membrane; Cell projection; Ciliopathy; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Endoplasmic reticulum; Glycoprotein; Joubert syndrome; Meckel syndrome; Membrane; Mental retardation; Nephronophthisis; Obesity; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 995 AA 
Protein Sequence
MATRGGAGVA MAVWSLLSAR AVTAFLLLFL PRFLQAQTFS FPFQQPEKCD NNQYFDISAL 60
SCVPCGANQR QDARGTSCVC LPGFQMISNN GGPAIICKKC PENMKGVTED GWNCISCPSD 120
LTAEGKCHCP IGHILVERDI NGTLLSQATC ELCDGNENSF MVVNALGDRC VRCEPTFVNT 180
SRSCACSEPN ILTGGLCFSS TGNFPLRRIS AARYGEVGMS LTSEWFAKYL QSSAAACWVY 240
ANLTSCQALG NMCVMNMNSY DFATFDACGL FQFIFENTAG LSTVHSISFW RQNLPWLFYG 300
DQLGLAPQVL SSTSLPTNFS FKGENQNTKL KFVAASYDIR GNFLKWQTLE GGVLQLCPDT 360
ETRLNAAYSF GTTYQQNCEI PISKILIDFP TPIFYDVYLE YTDENQHQYI LAVPVLNLNL 420
QHNKIFVNQD SNSGKWLLTR RIFLVDAVSG RENDLGTQPR VIRVATQISL SVHLVPNTIN 480
GNIYPPLITI AYSDIDIKDA NSQSVKVSFS VTYEMDHGEA HVQTDIALGV LGGLAVLASL 540
LKTAGWKRRI GSPMIDLQTV VKFLVYYAGD LANVFFIITV GTGLYWLIFF KAQKSVSVLL 600
PMPIQEERFV TYVGCAFALK ALQFLHKLIS QITIDVFFID WERPKGKVLK AVEGEGGVRS 660
ATVPVSIWRT YFVANEWNEI QTVRKINSLF QVLTVLFFLE VVGFKNLALM DSSSSLSRNP 720
PSYIAPYSCI LRYAVSAALW LAIGIIQVVF FAVFYERFIE DKIRQFVDLC SMSNISVFLL 780
SHKCFGYYIH GRSVHGHADT NMEEMNMNLK REAENLCSQR GLVPNTDGQT FEIAISNQMR 840
QHYDRIHETL IRKNGPARLL SSSASTFEQS IKAYHMMNKF LGSFIDHVHK EMDYFIKDKL 900
LLERILGMEF MEPMEKSIFY NDEGYSFSSV LYYGNEATLL IFDLLFFCVV DLACQNFILA 960
SFLTYLQQEI FRYIRNTVGQ KNLASKTLVD QRFLI 995 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0060170; C:cilium membrane; IDA:UniProtKB.
 GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005932; C:microtubule basal body; IEA:UniProtKB-SubCell.
 GO:0036038; C:TCTN-B9D complex; ISS:UniProtKB.
 GO:0042384; P:cilium assembly; IMP:UniProtKB.
 GO:0030433; P:ER-associated protein catabolic process; IMP:UniProtKB.
 GO:0010826; P:negative regulation of centrosome duplication; IMP:UniProtKB. 
Interpro
 IPR009030; Growth_fac_rcpt_N_dom.
 IPR019170; Meckelin. 
Pfam
 PF09773; Meckelin 
SMART
  
PROSITE
  
PRINTS