| Tag | Content |
|---|
| CPLM ID | CPLM-006239 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA repair and recombination protein RAD54 |
Protein Synonyms/Alias | |
Gene Name | RAD54 |
Gene Synonyms/Alias | YGL163C; G1821 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 710 | DGTMSINKRQKLVDR | ubiquitination | [1] | | 808 | SSCVVDAKEDVERLF | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. Addition of RAD54 overcomes inhibition of DNA strand exchange by RAD51 bound to substrate dsDNA. Species preference in the RAD51 dissociation and DNA strand exchange assays underlines the importance of specific RAD54-RAD51 interactions. RAD51 is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange. |
Sequence Annotation | DOMAIN 322 504 Helicase ATP-binding.
DOMAIN 659 812 Helicase C-terminal.
NP_BIND 335 342 ATP (Potential).
MOTIF 21 25 Nuclear localization signal (Potential).
MOTIF 41 45 Nuclear localization signal (Potential).
MOTIF 455 458 DEGH box. |
Keyword | ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 898 AA |
Protein Sequence | MARRRLPDRP PNGIGAGERP RLVPRPINVQ DSVNRLTKPF RVPYKNTHIP PAAGRIATGS 60
DNIVGGRSLR KRSATVCYSG LDINADEAEY NSQDISFSQL TKRRKDALSA QRLAKDPTRL 120
SHIQYTLRRS FTVPIKGYVQ RHSLPLTLGM KKKITPEPRP LHDPTDEFAI VLYDPSVDGE 180
MIVHDTSMDN KEEESKKMIK STQEKDNINK EKNSQEERPT QRIGRHPALM TNGVRNKPLR 240
ELLGDSENSA ENKKKFASVP VVIDPKLAKI LRPHQVEGVR FLYRCVTGLV MKDYLEAEAF 300
NTSSEDPLKS DEKALTESQK TEQNNRGAYG CIMADEMGLG KTLQCIALMW TLLRQGPQGK 360
RLIDKCIIVC PSSLVNNWAN ELIKWLGPNT LTPLAVDGKK SSMGGGNTTV SQAIHAWAQA 420
QGRNIVKPVL IISYETLRRN VDQLKNCNVG LMLADEGHRL KNGDSLTFTA LDSISCPRRV 480
ILSGTPIQND LSEYFALLSF SNPGLLGSRA EFRKNFENPI LRGRDADATD KEITKGEAQL 540
QKLSTIVSKF IIRRTNDILA KYLPCKYEHV IFVNLKPLQN ELYNKLIKSR EVKKVVKGVG 600
GSQPLRAIGI LKKLCNHPNL LNFEDEFDDE DDLELPDDYN MPGSKARDVQ TKYSAKFSIL 660
ERFLHKIKTE SDDKIVLISN YTQTLDLIEK MCRYKHYSAV RLDGTMSINK RQKLVDRFND 720
PEGQEFIFLL SSKAGGCGIN LIGANRLILM DPDWNPAADQ QALARVWRDG QKKDCFIYRF 780
ISTGTIEEKI FQRQSMKMSL SSCVVDAKED VERLFSSDNL RQLFQKNENT ICETHETYHC 840
KRCNAQGKQL KRAPAMLYGD ATTWNHLNHD ALEKTNDHLL KNEHHYNDIS FAFQYISH 898 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:SGD. GO:0005634; C:nucleus; IDA:SGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0015616; F:DNA translocase activity; IDA:SGD. GO:0004386; F:helicase activity; IEA:UniProtKB-KW. GO:0006338; P:chromatin remodeling; IDA:SGD. GO:0032392; P:DNA geometric change; IDA:SGD. GO:0045003; P:double-strand break repair via synthesis-dependent strand annealing; TAS:SGD. GO:0030491; P:heteroduplex formation; IDA:SGD. GO:0032079; P:positive regulation of endodeoxyribonuclease activity; IDA:SGD. GO:0000722; P:telomere maintenance via recombination; IMP:SGD. |
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Pfam | |
SMART | |
PROSITE | |
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