UniProt Accession

 SEN54_HUMAN; Q7Z6J9; Q86WV3; Q86XE4; Q8N9H2 

Genbank Protein ID

 AK094466; BC047793; AC100787; BC053643 

Genbank Nucleotide ID

 BAC04362.1; AAH47793.1; AAH53643.1 

Protein Name

 tRNA-splicing endonuclease subunit Sen54 

Protein Synonyms/Alias

 SEN54 homolog; HsSEN54; tRNA-intron endonuclease Sen54 

Gene Name

 TSEN54 

Gene Synonyms/Alias

 SEN54 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
92	EEGFVELKSPAGKFW	ubiquitination	[1]
97	ELKSPAGKFWQTMGF	ubiquitination	[2]
340	DAESWCQKLNQRKEK	ubiquitination	[1]
382	CSSWREYKELLQRRQ	ubiquitination	[1]
489	VPDLCSLKRLSYQSG	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 267 267 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; mRNA processing; Nucleus; Phosphoprotein; Polymorphism; Pontocerebellar hypoplasia; Reference proteome; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 526 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IDA:UniProtKB. 

Interpro

 IPR024337; tRNA_splic_suSen54.
 IPR024336; tRNA_splic_suSen54_N. 

Pfam

 PF12928; tRNA_int_end_N2 

SMART

  

PROSITE

  

PRINTS