UniProt Accession

 AHPF_ECOLI; P35340; P77251; P77462 

Genbank Protein ID

 U82598; U00096; AP009048; D13187 

Genbank Nucleotide ID

 AAB40807.1; AAC73707.2; BAA35236.1; BAA02486.1 

Protein Name

 Alkyl hydroperoxide reductase subunit F 

Protein Synonyms/Alias

 Alkyl hydroperoxide reductase F52A protein 

Gene Name

 ahpF 

Gene Synonyms/Alias

 b0606; JW0599 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
11	TNMKTQLKAYLEKLT	acetylation	[1, 2]
16	QLKAYLEKLTKPVEL	acetylation	[1, 2]
19	AYLEKLTKPVELIAT	acetylation	[1, 2, 3]
32	ATLDDSAKSAEIKEL	acetylation	[2]
37	SAKSAEIKELLAEIA	acetylation	[2]
49	EIAELSDKVTFKEDN	acetylation	[2]
53	LSDKVTFKEDNSLPV	acetylation	[2, 4]
179	PAVFVNGKEFGQGRM	acetylation	[2]
194	TLTEIVAKIDTGAEK	acetylation	[2]
201	KIDTGAEKRAAEELN	acetylation	[2]
209	RAAEELNKRDAYDVL	acetylation	[2]
268	VPKTEGQKLAGALKV	acetylation	[1, 2]
314	TASGAVLKARSIIVA	acetylation	[1, 2]
325	IIVATGAKWRNMNVP	acetylation	[2]
354	HCDGPLFKGKRVAVI	acetylation	[2, 4]
399	ADQVLQDKLRSLKNV	acetylation	[2]
404	QDKLRSLKNVDIILN	acetylation	[2]
423	EVKGDGSKVVGLEYR	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [4] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein. 

Sequence Annotation

 NP_BIND 214 229 FAD (By similarity).
 NP_BIND 357 371 NAD or NADP (By similarity).
 NP_BIND 478 488 FAD (By similarity).
 MOD_RES 53 53 N6-acetyllysine.
 MOD_RES 354 354 N6-acetyllysine.
 DISULFID 345 348 Redox-active (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Disulfide bond; FAD; Flavoprotein; NAD; NADP; Oxidoreductase; Redox-active center; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 521 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0008785; F:alkyl hydroperoxide reductase activity; IGI:EcoliWiki.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0071949; F:FAD binding; IDA:EcoCyc.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0000302; P:response to reactive oxygen species; IEA:InterPro. 

Interpro

 IPR012081; Alkyl_hydroperoxide_Rdtase_suF.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR002109; Glutaredoxin.
 IPR008255; Pyr_nucl-diS_OxRdtase_2_AS.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom.
 IPR000103; Pyridine_nuc-diS_OxRdtase_2.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00070; Pyr_redox
 PF07992; Pyr_redox_2
 PF13192; Thioredoxin_3 

SMART

  

PROSITE

 PS51354; GLUTAREDOXIN_2
 PS00573; PYRIDINE_REDOX_2 

PRINTS

 PR00368; FADPNR.
 PR00469; PNDRDTASEII.