CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010551
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AP-2 complex subunit mu 
Protein Synonyms/Alias
 AP-2 mu chain; Adapter-related protein complex 2 mu subunit; Adaptor protein complex AP-2 subunit mu; Clathrin assembly protein complex 2 medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein 
Gene Name
 Ap2m1 
Gene Synonyms/Alias
 Clapm1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
139FITQQGIKSQHQTKEubiquitination[1]
145IKSQHQTKEEQSQITubiquitination[1]
213LSGMPECKFGMNDKIacetylation[2]
219CKFGMNDKIVIEKQGubiquitination[1]
224NDKIVIEKQGKGTADubiquitination[1]
238DETSKSGKQSIAIDDubiquitination[1]
256HQCVRLSKFDSERSIacetylation[3]
256HQCVRLSKFDSERSIubiquitination[1]
281LMRYRTTKDIILPFRubiquitination[1]
312VVIKSNFKPSLLAQKubiquitination[1]
339GVQVICMKGKAKYKAubiquitination[1]
345MKGKAKYKASENAIVubiquitination[1]
354SENAIVWKIKRMAGMubiquitination[1]
378ELLPTNDKKKWARPPubiquitination[1]
405GLKVRYLKVFEPKLNacetylation[3]
410YLKVFEPKLNYSDHDubiquitination[1]
420YSDHDVIKWVRYIGRacetylation[2]
420YSDHDVIKWVRYIGRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X- X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane- associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity). 
Sequence Annotation
 DOMAIN 170 434 MHD.
 BINDING 341 341 Phosphatidylinositol lipid headgroup (By
 BINDING 343 343 Phosphatidylinositol lipid headgroup (By
 BINDING 345 345 Phosphatidylinositol lipid headgroup (By
 BINDING 354 354 Phosphatidylinositol lipid headgroup (By
 BINDING 356 356 Phosphatidylinositol lipid headgroup (By
 MOD_RES 45 45 Phosphoserine (By similarity).
 MOD_RES 156 156 Phosphothreonine (By similarity).  
Keyword
 Cell membrane; Coated pit; Complete proteome; Direct protein sequencing; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 435 AA 
Protein Sequence
MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI ARTSFFHVKR 60
SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN IKNNFVLIYE LLDEILDFGY 120
PQNSETGALK TFITQQGIKS QHQTKEEQSQ ITSQVTGQIG WRREGIKYRR NELFLDVLES 180
VNLLMSPQGQ VLSAHVSGRV VMKSYLSGMP ECKFGMNDKI VIEKQGKGTA DETSKSGKQS 240
IAIDDCTFHQ CVRLSKFDSE RSISFIPPDG EFELMRYRTT KDIILPFRVI PLVREVGRTK 300
LEVKVVIKSN FKPSLLAQKI EVRIPTPLNT SGVQVICMKG KAKYKASENA IVWKIKRMAG 360
MKESQISAEI ELLPTNDKKK WARPPISMNF EVPFAPSGLK VRYLKVFEPK LNYSDHDVIK 420
WVRYIGRSGI YETRC 435 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0030141; C:secretory granule; TAS:MGI.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0006886; P:intracellular protein transport; TAS:MGI.
 GO:0016192; P:vesicle-mediated transport; TAS:MGI. 
Interpro
 IPR022775; AP_mu_sigma_su.
 IPR015629; Clathrin_AP50.
 IPR001392; Clathrin_mu.
 IPR008968; Clathrin_mu_C.
 IPR018240; Clathrin_mu_CS.
 IPR011012; Longin-like_dom. 
Pfam
 PF00928; Adap_comp_sub
 PF01217; Clat_adaptor_s 
SMART
  
PROSITE
 PS00990; CLAT_ADAPTOR_M_1
 PS00991; CLAT_ADAPTOR_M_2
 PS51072; MHD 
PRINTS
 PR00314; CLATHRINADPT.