UniProt Accession

 MTDC_MOUSE; P18155; Q3TMN4 

Genbank Protein ID

 J04627; M63445; M63415; M63439; M63440; M63441; M63442; M63443; M63444; AK076019; AK159680; AK165840; BC019511; S52980 

Genbank Nucleotide ID

 AAA39827.1; AAA39828.1; AAA39828.1; AAA39828.1; AAA39828.1; AAA39828.1; AAA39828.1; AAA39828.1; AAA39828.1; BAC36124.1; BAE35283.1; BAE38407.1; AAH19511.1 

Protein Name

 Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial 

Protein Synonyms/Alias

 NAD-dependent methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase 

Gene Name

 Mthfd2 

Gene Synonyms/Alias

 Nmdmc 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
152	CNAVSPDKDVDGFHV	acetylation	[1, 2]
286	VQDPVTAKPKLVGDV	acetylation	[2]
286	VQDPVTAKPKLVGDV	succinylation	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

  

Sequence Annotation

 MOD_RES 50 50 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Magnesium; Mitochondrion; Multifunctional enzyme; NAD; One-carbon metabolism; Oxidoreductase; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 350 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0000287; F:magnesium ion binding; IEA:Compara.
 GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IBA:RefGenome.
 GO:0004487; F:methylenetetrahydrofolate dehydrogenase (NAD+) activity; IBA:RefGenome.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IBA:RefGenome.
 GO:0042301; F:phosphate ion binding; IEA:Compara.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IBA:RefGenome.
 GO:0009408; P:response to heat; IEA:Compara.
 GO:0046653; P:tetrahydrofolate metabolic process; IEA:Compara. 

Interpro

 IPR016040; NAD(P)-bd_dom.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020867; THF_DH/CycHdrlase_CS.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 

Pfam

 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 

SMART

  

PROSITE

 PS00766; THF_DHG_CYH_1
 PS00767; THF_DHG_CYH_2 

PRINTS

 PR00085; THFDHDRGNASE.