UniProt Accession

 HAUS4_HUMAN; Q9H6D7; B7WP17; D3DS34; Q86T15; Q86T16; Q86U43; Q9NX59 

Genbank Protein ID

 AK026016; AK000431; BX247981; BX247995; BX248256; AL132780; CH471078; CH471078; CH471078; CH471078; CH471078; BC001916; BC002554 

Genbank Nucleotide ID

 BAB15322.1; BAA91161.1; CAD62315.1; CAD62328.1; CAD62584.1; EAW66207.1; EAW66206.1; EAW66209.1; EAW66210.1; EAW66213.1; AAH01916.1; AAH02554.1 

Protein Name

 HAUS augmin-like complex subunit 4 

Protein Synonyms/Alias

  

Gene Name

 HAUS4 

Gene Synonyms/Alias

 C14orf94 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
69	KEQAQAWKEVRLHKT	ubiquitination	[1]
75	WKEVRLHKTTWLRSE	ubiquitination	[1]
109	TNVTSEDKKFHETLE	ubiquitination	[1]
110	NVTSEDKKFHETLEQ	ubiquitination	[1]
173	QEVEEQLKKKCFTLL	ubiquitination	[1]
175	VEEQLKKKCFTLLCY	ubiquitination	[1]
201	VKAAKVWKLAEVLVG	ubiquitination	[1]
217	QQQCQDAKSQQKEQM	ubiquitination	[1]
221	QDAKSQQKEQMLLLE	ubiquitination	[1]
230	QMLLLEKKSAAYSQV	ubiquitination	[1]
255	LLQEHRLKTQSELDR	ubiquitination	[1]
339	EEFDRLVKEYTVLKQ	ubiquitination	[1]
345	VKEYTVLKQATENKR	acetylation	[2]
345	VKEYTVLKQATENKR	ubiquitination	[1]
351	LKQATENKRWALQEF	acetylation	[2]
360	WALQEFSKVYR****	ubiquitination	[1, 3, 4, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. 

Sequence Annotation

  

Keyword

 Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 363 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0070652; C:HAUS complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0051225; P:spindle assembly; IMP:UniProtKB. 

Interpro

 IPR026214; HAUS4. 

Pfam

  

SMART

  

PROSITE

  

PRINTS

 PR02090; HAUSAUGMINL4.