CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006953
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase RCK2 
Protein Synonyms/Alias
 CAM kinase-like protein kinase CLK1 
Gene Name
 RCK2 
Gene Synonyms/Alias
 CLK1; CMK3; YLR248W; L9672.6 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
38RSSGTNNKDVSQITSubiquitination[1]
431KIDTLTEKISRGEYTubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Serine/threonine-protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. 
Sequence Annotation
 DOMAIN 163 478 Protein kinase.
 NP_BIND 169 177 ATP (By similarity).
 REGION 493 506 Calmodulin-binding (Potential).
 ACT_SITE 313 313 Proton acceptor (By similarity).
 BINDING 201 201 ATP (By similarity).
 MOD_RES 46 46 Phosphoserine.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 187 187 Phosphoserine.
 MOD_RES 350 350 Phosphothreonine.
 MOD_RES 520 520 Phosphoserine.  
Keyword
 ATP-binding; Calmodulin-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 610 AA 
Protein Sequence
MLKIKALFSK KKPDQADLSQ ESKKPFKGKT RSSGTNNKDV SQITSSPKKS FQDKNIVQYP 60
SVVADDHHMK SLTDELVTTI DSDSSPSDNI TTENVETVTS VPAIDVHESS EGQLSSDPLI 120
SDESLSEQSE IISDIQDDST DDDNMEDEIP EKSFLEQKEL IGYKLINKIG EGAFSKVFRA 180
IPAKNSSNEF LTKNYKAVAI KVIKKADLSS INGDHRKKDK GKDSTKTSSR DQVLKEVALH 240
KTVSAGCSQI VAFIDFQETD SYYYIIQELL TGGEIFGEIV RLTYFSEDLS RHVIKQLALA 300
VKHMHSLGVV HRDIKPENLL FEPIEFTRSI KPKLRKSDDP QTKADEGIFT PGVGGGGIGI 360
VKLADFGLSK QIFSKNTKTP CGTVGYTAPE VVKDEHYSMK VDMWGIGCVL YTMLCGFPPF 420
YDEKIDTLTE KISRGEYTFL KPWWDEISAG AKNAVAKLLE LEPSKRYDID QFLDDPWLNT 480
FDCLPKEGES SQKKAGTSER RHPHKKQFQL FQRDSSLLFS PAAVAMRDAF DIGNAVKRTE 540
EDRMGTRGGL GSLAEDEELE DSYSGAQGDE QLEQNMFQLT LDTSTILQRR KKVQENDVGP 600
TIPISATIRE 610 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0034599; P:cellular response to oxidative stress; IMP:SGD.
 GO:0007231; P:osmosensory signaling pathway; IMP:SGD.
 GO:0040020; P:regulation of meiosis; IGI:SGD. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS