UniProt Accession

 ENAH_HUMAN; Q8N8S7; Q502W5; Q5T5M7; Q5VTQ9; Q5VTR0; Q9NVF3; Q9UFB8 

Genbank Protein ID

 AY345143; AF519769; AL591380; AC092811; AL356216; AL591380; AC092811; AL356216; AL356216; AC092811; AL356216; AL591380; AC092811; AL356216; AL591380; AC092811; BC065238; BC095481; AK001635; AK096246; AL133059 

Genbank Nucleotide ID

 AAR04685.1; AAQ08487.1; CAH71475.1; CAH71475.1; CAH71475.1; CAH71476.1; CAH71476.1; CAH71476.1; CAI22018.1; CAI22018.1; CAI22019.1; CAI22019.1; CAI22019.1; CAI22020.1; CAI22020.1; CAI22020.1; AAH65238.1; AAH95481.1; BAA91799.1; BAC04736.1; CAB61384.1 

Protein Name

 Protein enabled homolog 

Protein Synonyms/Alias

  

Gene Name

 ENAH 

Gene Synonyms/Alias

 MENA 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
69	CAIPKGLKYNQATQT	ubiquitination	[1, 2]
461	RRRRIAEKGSTIETE	acetylation	[3]
574	RKELTKLKEELIDAI	ubiquitination	[1]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity). 

Sequence Annotation

 DOMAIN 1 111 WH1.
 REPEAT 156 160 1.
 REPEAT 161 165 2.
 REPEAT 166 170 3.
 REPEAT 171 175 4.
 REPEAT 176 180 5.
 REPEAT 181 185 6.
 REPEAT 186 190 7.
 REPEAT 191 195 8.
 REPEAT 196 200 9.
 REGION 156 200 9 X 5 AA tandem repeats of [LMQ]-E-[QR]-
 REGION 391 588 EVH2.
 REGION 391 411 EVH2 block A.
 REGION 442 459 EVH2 block B.
 REGION 554 588 EVH2 block C.
 MOTIF 400 403 KLKR.
 MOD_RES 125 125 Phosphoserine.
 MOD_RES 265 265 Phosphoserine; by PKA (By similarity).
 MOD_RES 506 506 Phosphoserine.
 MOD_RES 508 508 Phosphoserine.  

Keyword

 3D-structure; Actin-binding; Alternative splicing; Cell junction; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; SH3-binding; Synapse. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 591 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0001725; C:stress fiber; IEA:Compara.
 GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
 GO:0008154; P:actin polymerization or depolymerization; IEA:Compara.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0046907; P:intracellular transport; IEA:InterPro.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0050852; P:T cell receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR000697; EVH1.
 IPR011993; PH_like_dom.
 IPR000156; Ran_bind_dom.
 IPR014885; VASP_tetra. 

Pfam

 PF08776; VASP_tetra
 PF00568; WH1 

SMART

 SM00160; RanBD
 SM00461; WH1 

PROSITE

 PS50229; WH1 

PRINTS