UniProt Accession

 BNI3L_HUMAN; O60238; Q5JW63; Q8NF87 

Genbank Protein ID

 AB004788; AF079221; AF067396; AF536326; AF452712; AF255051; BT019501; AL132665; BC001559; BC009603 

Genbank Nucleotide ID

 BAA28692.1; AAC27723.1; AAD03589.1; AAN04051.1; AAL50978.1; AAF70290.1; AAV38308.1; CAI46217.1; AAH01559.1; AAH09603.1 

Protein Name

 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like 

Protein Synonyms/Alias

 Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIP3-like protein X; NIP3L 

Gene Name

 BNIP3L 

Gene Synonyms/Alias

 BNIP3A; BNIP3H; NIX 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
135	EKEVEALKKSADWVS	ubiquitination	[1]
136	KEVEALKKSADWVSD	ubiquitination	[2]
154	RPENIPPKEFHFRHP	ubiquitination	[1, 3]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. 

Sequence Annotation

 MOTIF 126 148 BH3.
 MOD_RES 62 62 Phosphoserine (By similarity).
 MOD_RES 63 63 Phosphoserine (By similarity).
 MOD_RES 117 117 Phosphoserine (By similarity).
 MOD_RES 118 118 Phosphoserine (By similarity).
 MOD_RES 120 120 Phosphoserine.  

Keyword

 Apoptosis; Complete proteome; Endoplasmic reticulum; Host-virus interaction; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 219 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031224; C:intrinsic to membrane; TAS:UniProtKB.
 GO:0005741; C:mitochondrial outer membrane; IMP:UniProtKB.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0005521; F:lamin binding; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051607; P:defense response to virus; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IDA:UniProtKB.
 GO:0035694; P:mitochondrial protein catabolic process; IMP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR010548; BNIP3. 

Pfam

 PF06553; BNIP3 

SMART

  

PROSITE

 PS01259; BH3 

PRINTS