UniProt Accession

 ENOG_HUMAN; P09104; Q96J33 

Genbank Protein ID

 X13120; X14327; M36768; M22349; X51956; U47924; BT007383; BC002745 

Genbank Nucleotide ID

 CAA31512.1; CAA32505.1; AAA52388.1; AAB59554.1; CAA36215.1; AAB51320.1; AAP36047.1; AAH02745.1 

Protein Name

 Gamma-enolase 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE 

Gene Name

 ENO2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MSIEKIWAREIL	ubiquitination	[1, 2]
28	EVDLYTAKGLFRAAV	ubiquitination	[1]
60	DKQRYLGKGVLKAVD	ubiquitination	[2]
193	AEVYHTLKGVIKDKY	acetylation	[3]
193	AEVYHTLKGVIKDKY	ubiquitination	[2]
197	HTLKGVIKDKYGKDA	acetylation	[3]
199	LKGVIKDKYGKDATN	ubiquitination	[2]
256	SEFYRDGKYDLDFKS	ubiquitination	[2]
262	GKYDLDFKSPTDPSR	ubiquitination	[2]
335	IERAVEEKACNCLLL	ubiquitination	[2]
358	TEAIQACKLAQENGW	ubiquitination	[2]
406	CRSERLAKYNQLMRI	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). 

Sequence Annotation

 REGION 370 373 Substrate binding (By similarity).
 ACT_SITE 210 210 Proton donor (By similarity).
 ACT_SITE 343 343 Proton acceptor (By similarity).
 METAL 245 245 Magnesium.
 METAL 293 293 Magnesium.
 METAL 318 318 Magnesium.
 BINDING 158 158 Substrate (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 293 293 Substrate (By similarity).
 BINDING 318 318 Substrate (By similarity).
 BINDING 394 394 Substrate (By similarity).
 MOD_RES 25 25 Phosphotyrosine (By similarity).
 MOD_RES 26 26 Phosphothreonine.
 MOD_RES 44 44 Phosphotyrosine.
 MOD_RES 80 80 Phosphoserine (By similarity).  

Keyword

 3D-structure; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 434 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043204; C:perikaryon; IEA:Compara.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0001917; C:photoreceptor inner segment; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; TAS:ProtInc.
 GO:0006094; P:gluconeogenesis; TAS:Reactome.
 GO:0006096; P:glycolysis; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.