CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040825
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Insulin-like growth factor 2 mRNA-binding protein 2 
Protein Synonyms/Alias
  
Gene Name
 IGF2BP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
77EVDYSVSKKLRSRKIubiquitination[1]
145EAKIAMEKLSGHQFEubiquitination[2]
227LTIKNITKQTQSRVDubiquitination[1, 3]
276QKEADETKLAEEIPLubiquitination[1, 3]
284LAEEIPLKILAHNGLubiquitination[1, 2, 3, 4]
468RFAGASIKIAPAEGPubiquitination[1, 2, 3, 4, 5]
493GPPEAQFKAQGRIFGubiquitination[1, 3, 4, 6, 7, 8]
503GRIFGKLKEENFFNPubiquitination[1, 2, 3]
536RVIGKGGKTVNELQNubiquitination[3]
594QVKQQEQKYPQGVASubiquitination[1, 2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 605 AA 
Protein Sequence
MMNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW AIRAIETLSG 60
KVELHGKIME VDYSVSKKLR SRKIQIRNIP PHLQWEVLDG LLAQYGTVEN VEQVFAFSLV 120
NTDTETAVVN VTYATREEAK IAMEKLSGHQ FENYSFKISY IPDEEVSSPS PPQRAQRGDH 180
SSREQGHAPG GTSQARQIDF PLRILVPTQF VGAIIGKEGL TIKNITKQTQ SRVDIHRKEN 240
SGAAEKPVTI HATPEGTSEA CRMILEIMQK EADETKLAEE IPLKILAHNG LVGRLIGKEG 300
RNLKKIEHET GTKITISSLQ DLSIYNPERT ITVKGTVEAC ASAEIEIMKK LREAFENDML 360
AVNQQANLIP GLNLSALGIF STGLSVLSPP AGPRGAPPAA PYHPFTTHSG YFSSLYPHHQ 420
FGPFPHHHSY PEQEIVNLFI PTQAVGAIIG KKGAHIKQLA RFAGASIKIA PAEGPDVSER 480
MVIITGPPEA QFKAQGRIFG KLKEENFFNP KEEVKLEAHI RVPSSTAGRV IGKGGKTVNE 540
LQNLTSAEVI VPRDQTPDEN EEVIVRIIGH FFASQTAQRK IREIVQQVKQ QEQKYPQGVA 600
SQRSK 605 
Gene Ontology
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro. 
Interpro
 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00013; KH_1
 PF00076; RRM_1 
SMART
 SM00322; KH
 SM00360; RRM 
PROSITE
 PS50084; KH_TYPE_1
 PS50102; RRM 
PRINTS