CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013209
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phospho-beta-glucosidase BglA 
Protein Synonyms/Alias
 Phospho-beta-glucosidase A 
Gene Name
 bglA 
Gene Synonyms/Alias
 bglD; yqfC; b2901; JW2869 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
10VKKLTLPKDFLWGGAacetylation[1]
29QVEGGWNKGGKGPSIacetylation[1]
53GVPREITKEVLPGKYacetylation[1]
59TKEVLPGKYYPNHEAacetylation[1]
74VDFYGHYKEDIKLFAacetylation[1]
78GHYKEDIKLFAEMGFacetylation[1]
112QPNEEGLKFYDDMFDacetylation[1]
152YGSWTNRKVVDFFVRacetylation[1]
295ERRGFNIKMEDGDLDacetylation[1]
449YGFIYVNKHDDGTGDacetylation[1]
469KKSFNWYKEVIASNGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the hydrolysis of phosphorylated beta- glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p- nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin), with the exception of phosphorylated salicin, and a low affinity for phosphorylated beta-methyl-glucoside. Apparently, it has only a very limited role in the utilization of external beta-glucosides. 
Sequence Annotation
 ACT_SITE 180 180 Proton donor (Potential).
 ACT_SITE 377 377 Nucleophile (By similarity).  
Keyword
 3D-structure; Complete proteome; Glycosidase; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 479 AA 
Protein Sequence
MIVKKLTLPK DFLWGGAVAA HQVEGGWNKG GKGPSICDVL TGGAHGVPRE ITKEVLPGKY 60
YPNHEAVDFY GHYKEDIKLF AEMGFKCFRT SIAWTRIFPK GDEAQPNEEG LKFYDDMFDE 120
LLKYNIEPVI TLSHFEMPLH LVQQYGSWTN RKVVDFFVRF AEVVFERYKH KVKYWMTFNE 180
INNQRNWRAP LFGYCCSGVV YTEHENPEET MYQVLHHQFV ASALAVKAAR RINPEMKVGC 240
MLAMVPLYPY SCNPDDVMFA QESMRERYVF TDVQLRGYYP SYVLNEWERR GFNIKMEDGD 300
LDVLREGTCD YLGFSYYMTN AVKAEGGTGD AISGFEGSVP NPYVKASDWG WQIDPVGLRY 360
ALCELYERYQ RPLFIVENGF GAYDKVEEDG SINDDYRIDY LRAHIEEMKK AVTYDGVDLM 420
GYTPWGCIDC VSFTTGQYSK RYGFIYVNKH DDGTGDMSRS RKKSFNWYKE VIASNGEKL 479 
Gene Ontology
 GO:0008706; F:6-phospho-beta-glucosidase activity; IDA:EcoCyc.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. 
Interpro
 IPR001360; Glyco_hydro_1.
 IPR018120; Glyco_hydro_1_AS.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00232; Glyco_hydro_1 
SMART
  
PROSITE
 PS00572; GLYCOSYL_HYDROL_F1_1
 PS00653; GLYCOSYL_HYDROL_F1_2 
PRINTS
 PR00131; GLHYDRLASE1.