UniProt Accession

 MAGD1_HUMAN; Q9Y5V3; Q5VSH6; Q8IZ84; Q8WY92; Q9H352; Q9HBT4; Q9UF36 

Genbank Protein ID

 AF217963; AF258554; AF300328; AL929410; AL929410; BC014070; BC032473; AF124440; AL133628; AF132205 

Genbank Nucleotide ID

 AAG09704.1; AAG23757.1; AAQ14483.1; CAH71560.1; CAH71561.1; AAH14070.1; AAH32473.1; AAD31421.1; CAB63752.1; AAG35551.1 

Protein Name

 Melanoma-associated antigen D1 

Protein Synonyms/Alias

 MAGE tumor antigen CCF; MAGE-D1 antigen; Neurotrophin receptor-interacting MAGE homolog 

Gene Name

 MAGED1 

Gene Synonyms/Alias

 NRAGE; PP2250; PRO2292 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
135	ARPKSAFKVQNATTK	ubiquitination	[1]
142	KVQNATTKGPNGVYD	ubiquitination	[1, 2, 3]
157	FSQAHNAKDVPNTQP	ubiquitination	[1, 3]
165	DVPNTQPKAAFKSQN	ubiquitination	[1]
169	TQPKAAFKSQNATPK	ubiquitination	[1]
176	KSQNATPKGPNAAYD	ubiquitination	[1]
197	TGELAANKSEMAFKA	ubiquitination	[1]
203	NKSEMAFKAQNATTK	ubiquitination	[1, 2, 4]
210	KAQNATTKVGPNATY	ubiquitination	[1, 3, 4, 5]
234	DLANSRPKTPFKAWN	ubiquitination	[1, 3, 5]
238	SRPKTPFKAWNDTTK	ubiquitination	[1, 2, 3, 4]
245	KAWNDTTKAPTADTQ	ubiquitination	[1, 2, 3, 5]
307	IRGKRTRKINNLNVE	ubiquitination	[1, 2, 5]
536	ERANKLVKYLMLKDY	ubiquitination	[1, 2, 3, 4, 5]
541	LVKYLMLKDYTKVPI	ubiquitination	[1, 2, 4]
545	LMLKDYTKVPIKRSE	ubiquitination	[1]
549	DYTKVPIKRSEMLRD	ubiquitination	[1, 2]
578	RACFVLEKKFGIQLK	ubiquitination	[1, 2]
579	ACFVLEKKFGIQLKE	ubiquitination	[1, 3]
661	PLLGDLRKLLTYEFV	ubiquitination	[1, 2, 3, 4, 5]
669	LLTYEFVKQKYLDYR	ubiquitination	[1, 2, 5]
671	TYEFVKQKYLDYRRV	ubiquitination	[1, 3]
712	RFIAEVQKRDPRDWT	ubiquitination	[1, 2, 3, 4, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds NGFR/p75NTR and antagonizes its association with NTRK1/TrkA, inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate TP53/p53 transcriptional activity and inhibit cell proliferation. Enhances TP53 phosphorylation and accumulation. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. 

Sequence Annotation

 REPEAT 296 301 1.
 REPEAT 302 307 2.
 REPEAT 308 313 3.
 REPEAT 332 337 4.
 REPEAT 338 343 5.
 REPEAT 344 349 6.
 REPEAT 350 355 7.
 REPEAT 356 361 8.
 REPEAT 362 367 9.
 REPEAT 368 373 10.
 REPEAT 374 379 11.
 REPEAT 380 385 12.
 REPEAT 386 391 13.
 REPEAT 392 397 14.
 REPEAT 398 403 15.
 REPEAT 404 409 16.
 REPEAT 410 415 17.
 REPEAT 416 421 18.
 REPEAT 422 427 19.
 REPEAT 428 432 20; approximate.
 REPEAT 433 438 21.
 REPEAT 439 444 22.
 DOMAIN 471 669 MAGE.
 REGION 296 444 22 X 6 AA tandem repeats of W-[PQ]-X-P-X-
 MOD_RES 92 92 Phosphotyrosine.  

Keyword

 Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Tumor antigen; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 778 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; IEA:Compara.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0050680; P:negative regulation of epithelial cell proliferation; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0090190; P:positive regulation of branching involved in ureteric bud morphogenesis; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; TAS:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; TAS:UniProtKB. 

Interpro

 IPR002190; MAGE. 

Pfam

 PF01454; MAGE 

SMART

  

PROSITE

 PS50838; MAGE 

PRINTS